Mice carrying Tg5Bir have increased growth rates and high serum human growth hormone levels. The use of a shorter promoter including only nucleotides -596 to +21 drives anomalous expression in small intestinal crypts, colon and renal proximal tubular epithelial cells. The use of this longer promoter (from nucleotides -4000 to +21) dampens this anomalous expression at a younger age, although regulatory sequence beyond this is also required for proper control of expression. (Sweetser et al., 1988; Hauft et al., 1989; Cohn et al., 1991; Roth et al., 1991.)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice carrying Tg5Bir have increased growth rates and high serum human growth hormone levels. The use of a shorter promoter including only nucleotides -596 to +21 drives anomalous expression in small intestinal crypts, colon and renal proximal tubular epithelial cells. The use of this longer promoter (from nucleotides -4000 to +21) dampens this anomalous expression at a younger age, although regulatory sequence beyond this is also required for proper control of expression. (Sweetser et al., 1988; Hauft et al., 1989; Cohn et al., 1991; Roth et al., 1991.)
Nucleotides -4000 to +21 of the promoter of liver fatty acid binding protein from rat was placed upstream of human growth hormone sequence and injected into B/6 x LT/Sv F1 or B6SJL x B6SJL F2 oocytes. This line derived from the one founder that had PUC vector sequence integrated as well, due to an incomplete HindIII digest of the pLFhGH4 construct. (Sweetser et al., 1988)
Expressed Gene | GH1, growth hormone 1, human |
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Site of Expression |
Allele Name | transgene insertion 5, Edward H Birkenmeier |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | L-FABP(-4000 to +21)/hGH transgene; long promoter L-FABP-hGH plus pUC; Tg(L-FABP, L-GH)5Bir |
Gene Symbol and Name | Tg(Fabp1-GH1)5Bir, transgene insertion 5, Edward H Birkenmeier |
Gene Synonym(s) | |
Promoter | Fabp1, fatty acid binding protein 1, rat |
Expressed Gene | GH1, growth hormone 1, human |
Strain of Origin | (C57BL/6J x LT/Sv)F1 |
Chromosome | UN |
Molecular Note | This transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -4000 to +21 from the rat liver fatty-acid binding protein gene; the insertion also contains the pUC13 vector. Mice of this line (from founder G0#1) have ~66 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal intestinal expression gradient, highest in the proximal small intestine and declining progressively, though less rapidly for hGH than for Fabp1, through the distal colon. This Fabp1 5' regulatory segment suppresses the expression observed in colon and kidney of mice bearing similar transgenes with a shorter promoter (See Tg(Fabp1-GH1)7Bir, Tg(Fabp1-GH1)10Bir). |
When using the STOCK Tg(Fabp1-GH1)5Bir/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #001400 in your Materials and Methods section.
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