STOCK Tg(Fabp1-GH1)5Bir/J

Stock number: 001400
Research areas: Cell Biology Research, Developmental Biology Research, Endocrine Deficiency Research, Internal/Organ Research, Research Tools

Detailed description

Mice carrying Tg5Bir have increased growth rates and high serum human growth hormone levels. The use of a shorter promoter including only nucleotides -596 to +21 drives anomalous expression in small intestinal crypts, colon and renal proximal tubular epithelial cells. The use of this longer promoter (from nucleotides -4000 to +21) dampens this anomalous expression at a younger age, although regulatory sequence beyond this is also required for proper control of expression. (Sweetser et al., 1988; Hauft et al., 1989; Cohn et al., 1991; Roth et al., 1991.)

Development

Nucleotides -4000 to +21 of the promoter of liver fatty acid binding protein from rat was placed upstream of human growth hormone sequence and injected into B/6 x LT/Sv F1 or B6SJL x B6SJL F2 oocytes. This line derived from the one founder that had PUC vector sequence integrated as well, due to an incomplete HindIII digest of the pLFhGH4 construct. (Sweetser et al., 1988)

Allele

Symbol: Tg(Fabp1-GH1)5Bir
Name: transgene insertion 5, Edward H Birkenmeier
MGI accession ID: MGI:3800317
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Fabp1-GH1)5Bir
Marker name: transgene insertion 5, Edward H Birkenmeier
Chromosome: UN
Strain of origin: (C57BL/6J x LT/Sv)F1
Expressed genes: GH1,growth hormone 1,human
Molecular note: This transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -4000 to +21 from the rat liver fatty-acid binding protein gene; the insertion also contains the pUC13 vector. Mice of this line (from founder G0#1) have ~66 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal intestinal expression gradient, highest in the proximal small intestine and declining progressively, though less rapidly for hGH than for Fabp1, through the distal colon. This Fabp1 5' regulatory segment suppresses the expression observed in colon and kidney of mice bearing similar transgenes with a shorter promoter (See Tg(Fabp1-GH1)7Bir, Tg(Fabp1-GH1)10Bir).