STOCK Mgrn1^md Igl-1^b Igl1-r^lo/J

Stock number: 001277
Research areas: Dermatology Research, Immunology, Inflammation and Autoimmunity Research

Detailed description

Mice of this stock carry the “low” allele of the Igl1-r gene, which exhibit reduced levels of IGL1, along with the immunoglobulin lambda chain b variant allele (Igl-1^b ) and mahoganoid (Mgrn1^md).

Development

Mahoganoid (Mgrn1^md) arose spontaneously on strain C3H/HeJ at the Jackson Laboratory in 1957. The Igl-1^b allele at the immunoglobulin lamba chain cluster 1 locus is found in strains SJL, YBR, BSVS and others and the Igl1-r^lo allele occurs in strains SJL, BSVS and others. In 1972 Dr. M. C. Green received the fused phalanges (Fbn2^fp) stock from Dr. Katrina Hummel. Fused phalanges arose spontaneously in strain C3HeB/FeHu. The stock Dr. Green received had been crossed once to C57BL/6J and it was then brother x sister bred to F20 in 1976. At F18 the stock was given to Dr. Muriel Davisson and it was continued to be inbred to F21 when a fused phalanges male was outcrossed to a homozygous mahoganoid (Mgrn1^md) female. This mahoganoid female was the N2 offspring of a backcross that began with a mating of C57BL/6J x C3H/He-Mgrn1^md/+ from which an F1 was bred back to a C3H/HeJ-Mgrn1^md/Mgrn1^md, which was at F69. The N2 homozygous female resulting from this breeding was bred with the F21 fused phalanges male and their offspring were bred brother x sister to F20. At that point, a mahoganoid male was outcrossed to an Lyst^bg homozygous female on a congenic SJL/J background at generation N12F4 from Dr. John Roths. The resulting stock was sibling mated and a homozygous mahoganoid female was backcrossed to an SJL/J male. The stock was then bred as homozygous for mahoganoid (Mgrn1^md),Igl-1^b and Igl1-r^lo all on chromosome 16. It was cryopreserved in 1991 by mating homozygotes at F27 and F28.

Allele

Symbol: Mgrn1^md
Name: mahoganoid
MGI accession ID: MGI:1856070
Generation method: Spontaneous
Marker symbol: Mgrn1
Marker name: mahogunin, ring finger 1
Chromosome: 16
Strain of origin: C3H/HeJ
Molecular note: The mutation in md has been attributed to a 5 kb retroviral insertion located in the intronic region 5' of exon 12. Northern analysis and RT-PCR indicate that the expression of transcript is greatly reduced in these mice.
General note: The original md mutation arose in the C3H/HeJ strain. This gene is similar to mahogany (Atrn^mg) in its effects on coat color, but causes less darkening of the back, ears, and tail of agouti mice (J:13379)(J:13791).

Allele

Symbol: Igl-1^b
Name: b variant
MGI accession ID: MGI:1858046
Generation method: Not Applicable
Marker symbol: Igl-1
Marker name: immunoglobulin lambda chain, cluster 1
Chromosome: 16
Strain of origin: Not Applicable

Allele

MGI accession ID: MGI:96531
Marker symbol: Igl1-r
Marker name: immunoglobulin lambda chain regulator
Chromosome: 16