C3H/HeJ-Atp2b2^dfw/J

Stock number: 001276
Product name: deaf waddler
Research areas: Cell Biology Research, Neurobiology Research, Sensorineural Research

Detailed description

Mice homozygous for the deaf waddler spontaneous mutation (Atp2b2^dfw) are deaf, walk with a hesitant, wobbly gait, and bob their heads. Both sexes are fertile.

Development

The mutation deaf waddler (Atp2b2^dfw) arose spontaneously at The Jackson Laboratory in 1979 in the strain C3H/HeJ when that inbred was at F160. This mutant sub-line was bred to homozygosity and maintained by sibling mating homozgyotes until F12 when a homozygous male was backcrossed to a C3H/HeJ female. The strain was then maintained by sibling mating with forced heterozygosity. It was cryopreserved beginning in 1991 from the breeding of heterozygotes and homozygotes, with the females at generation F24 to F30 and the males at generation F23 to F25.

Allele

Symbol: Atp2b2^dfw
Name: deaf waddler
MGI accession ID: MGI:1856799
Generation method: Spontaneous
Marker symbol: Atp2b2
Marker name: ATPase, Ca++ transporting, plasma membrane 2
Chromosome: 6
Strain of origin: C3H/HeJ
Molecular note: A G to A transition point mutation at position 847 is predicted to cause a glycine to serine substitution at position 283 in the encoded protein.