Overview

Also Known As:meander tail Jackson

These mice carry the meander tail-J (mea^J) spontaneous mutation along with the segregating diabetes (Lepr^db) and misty (Dock7^m) mutations.

Genetic overview

Genetic Background	Generation

Lepr^db

Allele Type	Gene Symbol	Gene Name
Spontaneous	Lepr	leptin receptor

mea^J

Allele Type	Gene Symbol	Gene Name
Spontaneous	mea	meander tail

Allele Type	Gene Symbol	Gene Name
Spontaneous	Dock7	dedicator of cytokinesis 7

Research Applications

Diabetes and Obesity Research
Developmental Biology Research
Neurobiology Research
Endocrine Deficiency Research
Immunology, Inflammation and Autoimmunity Research
Internal/Organ Research
Reproductive Biology Research
Mouse/Human Gene Homologs

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for the meander tail-J spontaneous mutation (mea^J) have variably shortened and kinked tails. At about two weeks an unsteadiness of gait, often amounting to a tremor, appears. Homozygous mutant mice are smaller and less vigorous than wild-type controls but are fertile. The anterior lobe of the cerebellum is disorganized. Purkinje cells are scattered, some cellular layers never form, and Bergmann glial processes are absent. The cytoarchitecture of the posterior lobe is normal, and the transition from normal to disorganized areas is sharply demarcated. This mutation occurred in the BKS.Cg-Lepr^db +/+ Dock7^m strain so it is also segregating for the diabetes (Lepr^db) and misty (Dock7^m) mutations. See strain description for Stock No. 000642 for more information.

Control Suggestions

Untyped from the colony
000662 C57BLKS/J

Additional Information

Considerations for Choosing Controls

Genetics

Lepr^db

Allele Symbol: Lepr^db

Allele Name	diabetes
Allele Type	Spontaneous
Allele Synonym(s)	db; db/db; Lep^db; Lepr-; Lepr^db-1J; leprdb
Gene Symbol and Name	Lepr, leptin receptor
Gene Synonym(s)
Strain of Origin	C57BLKS/J
Chromosome	4
General Note	Phenotypic Similarity to Human Syndrome: Gestational Diabetes J:219658
Molecular Note	An intronic G-to-T transversion in this allele created a donor splice site that causes abnormal splicing and the inclusion of 106 nt of intronic sequence in the transcript, leading to premature termination of the long cellular domain of the Ob-Rb splice form and loss of its signal transducing function.

mea^J

Allele Symbol: mea^J

Allele Name	meander tail Jackson
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	mea, meander tail
Gene Synonym(s)
Strain of Origin	BKS.Cg-Dock7^m +/+ Lepr^db
Chromosome	4
Molecular Note	This allele was identified from the results of a noncomplementation test with mea, meander tail. The molecular lesion is unknown.

Dock7^m

Allele Symbol: Dock7^m

Allele Name	misty
Allele Type	Spontaneous
Allele Synonym(s)	m
Gene Symbol and Name	Dock7, dedicator of cytokinesis 7
Gene Synonym(s)
Strain of Origin	DBA/J
Chromosome	4
Molecular Note	Crosses between mice homozygous for misty and for moonlight, which mapped to overlapping critical regions on Chr 4, demonstrated failure of the two mutations to complement one another. Once moonlight had been identified as a mutation of Dock7 (Dock7^mnlt), sequence analysis of this gene from misty mice revealed a retrotransposon LTR insertion following nucleotide 2045 (numbering from the A of the transcription initiation codon) that interrupts exon 18 and shifts the reading frame after codon 682 so that ten incorrect amino acids are incorporated into the protein before its premature termination.

Disease/Phenotype

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

non-alcoholic fatty liver disease

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Lepr^db related

Diabetes and Obesity Research
- Impaired Wound Healing
- Obesity With Diabetes
- Hyperinsulinemia
- Insulin Resistance
Endocrine Deficiency Research
- Pancreas Defects
- Adipose Defects
- Hypothalamus/Pituitary Defects
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency Associated with Other Defects
Internal/Organ Research
- Adipose Defects
Reproductive Biology Research
- Fertility Defects
Mouse/Human Gene Homologs
- obesity, morbid, with hypogonadism (rare)
Metabolism Research

Genotype: mea^J related

Developmental Biology Research
- Skeletal Defects
Neurobiology Research
- Ataxia (Movement) Defects
- Cerebellar Defects

Genotype: Dock7^m related

Dermatology Research
- Color and White Spotting Defects

Diabetes and Obesity Research
- Hyperglycemia
- Type 2 Diabetes (NIDDM)

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: mea^J/mea^J
involves: C57BLKS/J

behavior/neurological phenotype

abnormal gait

limbs/digits/tail phenotype

short tail

kinked tail

Genotype: Lepr^db/Lepr^db
B6.BKS(D)-Lepr/JOrlRj

cellular phenotype

increased hepatocyte apoptosis
- some hepatocyte apoptosis is seen in mice fed the standard diet and apoptosis is not increased by a high-calorie diet and even reduced after 3 months of a high-calorie diet

growth/size/body region phenotype

increased susceptibility to diet-induced obesity
- mice exhibit increased body weight when fed a high-calorie diet for 1 or 3 months

increased body weight
- mice exhibit increased body weight at 3 months, but not 1 month, of standard chow diet

homeostasis/metabolism phenotype

increased cholesterol level
- total cholesterol levels are increased in mice fed a high-calorie diet for 1 month and increased further when fed for 3 months

increased susceptibility to diet-induced obesity
- mice exhibit increased body weight when fed a high-calorie diet for 1 or 3 months

increased circulating ketone body level
- plasma beta-hydroxybutyrate levels are greatly increased after 3 months of the high-calorie diet

immune system phenotype

liver inflammation
- hepatic necroinflammation is not prominent, however it is aggravated after 1 month of high-calorie diet compared to standard diet and tends to alleviate with time
- non-alcoholic steatohepatitis is seen occasionally after 3 months of a standard diet and is seen in 3 of 9 mice after 3 months of the high-calorie diet

liver/biliary system phenotype

hepatic steatosis
- mice develop steatosis, with steatosis mainly localized in the centrilobular and mediolobular areas; fatty change is milder than in Lep^ob homozygotes
- severity of steatosis is enhanced by the high-calorie diet after 1 month, but is subsequently reduced after 3 months on this diet

liver inflammation
- non-alcoholic steatohepatitis is seen occasionally after 3 months of a standard diet and is seen in 3 of 9 mice after 3 months of the high-calorie diet
- hepatic necroinflammation is not prominent, however it is aggravated after 1 month of high-calorie diet compared to standard diet and tends to alleviate with time

macrovesicular hepatic steatosis
- macrovacuolar steatosis is similar to that seen in Lep^ob homozygotes

increased hepatocyte apoptosis
- some hepatocyte apoptosis is seen in mice fed the standard diet and apoptosis is not increased by a high-calorie diet and even reduced after 3 months of a high-calorie diet

microvesicular hepatic steatosis
- microvesicular steatosis is more frequently seen than in Lep^ob homozygotes

liver fibrosis
- some portal and perisinusoidal fibrosis is seen in standard diet fed mice at 3 months and is increased when mice are fed the high-calorie diet

References

Additional References

Chen H; Charlat O; Tartaglia LA; Woolf EA; Weng X; Ellis SJ; Lakey ND; Culpepper J; Moore KJ; Breitbart RE; Duyk GM; Tepper RI; Morgenstern JP. 1996. Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell 84(3):491-5PubMed: 8608603 MGI: J:31324
Chua SC Jr; Chung WK; Wu-Peng XS; Zhang Y; Liu SM; Tartaglia L; Leibel RL. 1996. Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor [see comments] Science 271(5251):994-6PubMed: 8584938 MGI: J:31419
Hamre KM; Goldowitz D. 1996. Analysis of gene action in the meander tail mutant mouse: examination of cerebellar phenotype and mitotic activity of granule cell neuroblasts. J Comp Neurol 368(2):304-15PubMed: 8725309 MGI: J:32737

Additional - Dock7^m related

Additional - Lepr^db related

Additional - mea^J related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Restriction Enzyme Digest:Lepr
- Pyrosequencing:Lepr
- End Point Analysis:Lepr
- Genotyping resources and troubleshooting
Citation
When using the meander tail Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #001192 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous or Wild-type for mea. Heterozygous or Wild-typefor db, Heterozygous or Wild-type for m

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:meander tail Jackson

Genetic overview

Research Applications

Base Price

Detailed Description

Control Suggestions

Additional Information

Leprdb

Allele Symbol: Leprdb

meaJ

Allele Symbol: meaJ

Dock7m

Allele Symbol: Dock7m

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Leprdb related

Genotype: meaJ related

Genotype: Dock7m related

Mammalian Phenotype Terms by Genotype

Genotype: meaJ/meaJinvolves: C57BLKS/J

behavior/neurological phenotype

limbs/digits/tail phenotype

Genotype: Leprdb/LeprdbB6.BKS(D)-Lepr/JOrlRj

cellular phenotype

growth/size/body region phenotype

homeostasis/metabolism phenotype

immune system phenotype

liver/biliary system phenotype

References

Additional References

Additional - Dock7m related

Additional - Leprdb related

Additional - meaJ related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Lepr^db

Allele Symbol: Lepr^db

mea^J

Allele Symbol: mea^J

Dock7^m

Allele Symbol: Dock7^m

Genotype: Lepr^db related

Genotype: mea^J related

Genotype: Dock7^m related

Genotype: mea^J/mea^J
involves: C57BLKS/J

Genotype: Lepr^db/Lepr^db
B6.BKS(D)-Lepr/JOrlRj

Additional - Dock7^m related

Additional - Lepr^db related

Additional - mea^J related