The spontaneous mutation H2bm11 consists of an alteration in the nucleotide sequence of the K region and differs from that of H2-Kb by three nucleotides at codons 77 and 80.Read More +
From nucleotide sequencing of H2bm11 mRNA, this spontaneous mutation contains three nucleotide substitutions, AGC in place of GAC to change amino acid 77 from aspartic acid to serine and AAC in place of ACC to change amino acid 80 from threonine to asparagine. Geliebter and Nathenson (1988) showed that these sequence changes came from recombination with the C57BL/6ByKh H2b gene.
Melvold and Kohn identified the H2bm11 histoincompatibility in a (C57BL/6ByKh x BALB/cByKh)F1, and this mutation was backcrossed onto C57BL/6ByKh until N10 before sibling intercrossing to homozygosity. Embryos were cryopreserved at The Jackson Laboratory in 1997 from N10F38 homozygotes.
|Allele Name||b haplotype mutation 11|
|Gene Symbol and Name||H2, histocompatibility-2, MHC|
|Strain of Origin||(C57BL/6ByKh x BALB/cByKh)F1|
|Molecular Note||The bm11 mutation consists of three nucleotide substitutions that result in amino acid changes at codons 77 (aspartate to serine) and 80 (threonine to asparagine).|