The H2-Kbm3 histocompatibility variant differs from the parental H2-Kb sequence by 4 coding nucleotide substitutions, which result in 2 amino acid changes, D77S (GAC to AGC) and K89A (AAG to GCG), and one nucleotide substitution (G to A) 12 bases into intron 2 (Pease et al., 1993). This is predicted to impact the peptide binding sequence.
This histocompatibility variant was identified in the late 1960’s in the C57BL/6J colony held by Dr. Igor Egorov when he was still in Moscow. This colony was less than 5 years removed from the parental colony held at The Jackson Laboratory at that time (Geliebter and Nathenson, 1988). This mutant subline was cryopreserved at The Jackson Laboratory in 1996-97.
|Allele Name||b haplotype mutation 3|
|Allele Synonym(s)||H-2bd; M505|
|Gene Symbol and Name||H2, histocompatibility-2, MHC|
|Strain of Origin||C57BL/6Eg|
|Molecular Note||The bm3 mutation consists of four nucleotide substitutions resulting in amino acid changes at codon 77 (aspartate to serine) and 89 (lysine to alanine).|