B10.MBR-H2^bq1/SxEgJ

Stock number: 001154
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

This congenic strain carries a recombinant H2 haplotype consisting of K^b I^k S^k D^q, and is useful in producing specific anti-H-2K^b and anti-I-A^b antisera.

Detailed description

This congenic strain carries a recombinant H2 haplotype consisting of K^b I^k S^k D^q, and is useful in producing specific anti-H-2K^b and anti-I-A^b antisera.

Development

In 1979 Sachs et al. reported an MHC recombination event, named MBR, that arose when they backcrossed their B10.AKM strain (See Stock No. 000466) to C57BL/10 to minimize genetic drift. The F1 progeny of this backcross were intercrossed and 14 of the F2 offspring were tested via cytotoxicity assays for homozygosity of the AKM H2 haplotype. One of these F2 offspring was identified as a K^b I^k S^k D^q recombinant heterozygote and this haplotype was called bq1. This heterozygous female was outcrossed to a B10.S male to separate the bq1 haplotype from the b haplotype, and F1 offspring were intercrossed to generate mice on a B10 background homozygous for bq1. Repeated generations of sibling breeding resulted in the B10.MBR/Sx strain, also called B10.MBR-H2^bq1/Sx. This strain is also homozygous for Qa2^a, H2-Q1^a, and H2-T18^a. (Sachs et al., 1979; Hansen et al., 1980; Klein et al., 1983). In 1990 this strain was imported from David Sachs at Hazelton Laboratories into The Jackson Laboratory and embryos from sibling breeding were cryopreserved at N3G1F3 under stock# 001790. The available records indicate that some mice from this line were maintained by further sibling inbreeding in the laboratory of Dr Igor Egorov at The Jackson Laboratory, and this strain was maintained as a live distribution colony until early 2008. Embryos for this stock #001154 were frozen at approximately 73 filial generations of inbreeding beyond those frozen for the N3G1F3 embryos that were frozen under stock #001790.

Allele

Symbol: H2^bq1
Name: bq1 variant
MGI accession ID: MGI:7570170
Generation method: Spontaneous
Attributes: Not Applicable
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Marker synonyms: H-2; MHC-II
Chromosome: 17
Strain of origin: Not Applicable
Molecular note: This recombinant haplotype is K^b I^k S^k D^q and arose in a backcross of B10.AKM to C57BL/10.

Allele

MGI accession ID: MGI:95894
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Marker synonyms: H-2; MHC-II
Chromosome: 17