DBA/1LacJ

Stock number: 001140
Product name: DBA1 Lac
Strain synonyms: D1Lac
Research areas: Immunology, Inflammation and Autoimmunity Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

DBA/1LacJ mice have been widely used in studies of collagen-induced arthritis, although recent in-house studies suggest that the response to collagen induction in the DBA/1LacJ strain is not as robust as indicated by earlier studies. DBA substrains of mice, derived from DBA which is the oldest inbred strain of mice, have been widely used in many areas of research including age-related hearing loss.

Detailed description

Historical reports indicate DBA/1J and DBA/1LacJ mice immunized with type II collagen develop a severe polyarthritis mediated by an autoimmune response. Recent in-house studies suggest that the response to collagen induction in the DBA/1LacJ strain is not as robust as indicated by earlier studies. Arthritis models such as B10.RIII-H2^r H2-T18^b/(71NS)SnJ (Stock No. 000457) and BUB/BnJ (Stock No. 000653) can be used as alternatives to DBA/1LacJ. Similar to the human condition, mice with collagen-induced arthritis display synovitis and erosions of cartilage and bone. In addition, susceptibility in both humans and mice is linked to the expression of specific MHC class II molecules. DBA/1 mice show a low susceptibility to developing atherosclerotic aortic lesions (20 to 350 um2 atherosclerotic aortic lesions/aortic cross-section) following 14 weeks on an atherogenic diet (1.25% cholesterol, 0.5% cholic acid and 15% fat).

Development

The DBA inbred strain is the oldest of all inbred strains of mice. Dr. CC Little began inbreeding in 1909 from a mouse colony segregating for coat color. During 1929 and 1930 crosses were made among substrains, and several new substrains were established including DBA/1 and DBA/2. DBA/1 and DBA/2 differ at a large number of loci (including the MHC H2 haplotype) which most likely results from residual heterozygosity in the strain when the substrains were separated.

In 1955, mice were transfered from The Jackson Laboratory to Laboratory Animal Center (Lac), Carshalton, UK. In 1981, embryos from the Frozen Embryo Bank at Lac were sent to Les at The Jackson Laboratory.

Allele

Symbol: Cdh23^ahl
Name: age related hearing loss 1
MGI accession ID: MGI:3028349
Generation method: Spontaneous
Marker symbol: Cdh23
Marker name: cadherin 23 (otocadherin)
Chromosome: 10
Strain of origin: multiple strains
Molecular note: Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has shown this is caused by a G to A transition at coding nucleotide position 753 of Cdh23 (SNP rs257098870). This hypomorphic allele changes splice donor site G-GT to A-GT, causing frame skipping of exon 7. This is predicted to delete part of the 2nd and 3rd ectodomains and cause reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD-1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129P1/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129X1/SvJ, NOR/LtJ, A/J, C57BL/6, NOD/LtJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: 129S1/SvImJ, C3H/HeSnJ, I/LnJ, YBR/Ei, MRL/MpJ.