BKS.Cg-mea^2J Dock7^m/+ +/J

Stock number: 001049
Product name: meander tail 2 Jackson
Research areas: Dermatology Research, Developmental Biology Research, Neurobiology Research

Description

These mice carry a spontaneous remutation at the mea locus characterized by variably shortened and kinked tails. The strain is maintained in linkage with the misty coat color mutation (Dock7^m).

Detailed description

Mice homozygous for the meander tail 2J spontaneous mutation (mea^2J) have a phenotype that is very similar to the original meander tail (mea) and meander tail J mice (mea^J). Homozygous mutant mice have variably shortened and kinked tails. At about 2 weeks an unsteadiness of gait, often amounting to a tremor, appears. Homozygotes are smaller and less vigorous than wiltype controls but are fertile. The anterior lobe of the cerebellum is disorganized. Purkinje cells are scattered, some cellular layers never form, and Bergmann glial processes are absent. The cytoarchitecture of the posterior lobe is normal, and the transition from normal to disorganized areas is sharply demarcated. This strain is maintained in linkage with the misty coat color mutation (Dock7^m).

Allele

Symbol: mea^2J
Name: meander tail 2 Jackson
MGI accession ID: MGI:1856077
Generation method: Spontaneous
Marker symbol: mea
Marker name: meander tail
Chromosome: 4
Strain of origin: BKS.Cg-Dock7^m +/+ Lepr^db
Molecular note: This allele was identified from the results of a noncomplementation test with mea^J. The molecular lesion is unknown.

Allele

Symbol: Dock7^m
Name: misty
MGI accession ID: MGI:1856946
Generation method: Spontaneous
Marker symbol: Dock7
Marker name: dedicator of cytokinesis 7
Chromosome: 4
Strain of origin: DBA/J
Molecular note: Crosses between mice homozygous for misty and for moonlight, which mapped to overlapping critical regions on Chr 4, demonstrated failure of the two mutations to complement one another. Once moonlight had been identified as a mutation of Dock7 (Dock7^mnlt), sequence analysis of this gene from misty mice revealed a retrotransposon LTR insertion following nucleotide 2045 (numbering from the A of the transcription initiation codon) that interrupts exon 18 and shifts the reading frame after codon 682 so that ten incorrect amino acids are incorporated into the protein before its premature termination.