Both CAST/EiJ and CASA/RkJ (Stock No. 000735) were derived from wild mice trapped in Thailand. Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.Read More +
Both CAST/EiJ and CASA/RkJ (Stock No. 000735) were derived from wild mice trapped in Thailand. CAST is often combined with the common laboratory strains to generate F1 hybrids with high levels of heterozygosity for use in genetic mapping. Unlike the wild-derived strain SPRET, male F1 mice from a CAST cross are fertile.
Like CASA, CAST is resistant to flavivirus infection. The flavivirus family includes pathogens responsible for dengue, yellow fever and several forms of encephalitis. Most common laboratory mice are sensitive to flavivirus infection. Resistance/sensitivity is conferred through the oligoA synthase Oas1b locus. In a comparison of multiple strains, CASA and CAST exhibit reduced numbers of retinal ganglion cells as compared to common laboratory strains and other wild-derived strains. In a 2015 study comparing CAST/Ei and the other Collaborative Cross inbred strains (A/J, C3H/HeJ, C57BL/6J, DBA/2J, 129S1/SvImJ, NOD/LtJ, NZO/HlLtJ, and WSB/EiJ), dorsal root ganglion neurons from CAST/Ei mice demonstrate a significantly improved ability to regenerate axons in an inhibitory environment as determined by an increase in number of neurons with neurites and longer axonal process per neuron under both naive and pre-injured conditions. In addition, as compared to neurons from C57BL/6 mice, CAST/Ei neurons exhibit more extensive axonal regeneration in the spinal cord and optic nerve following injury and show greater sprouting following ischemic stroke.
Wild-derived mice are genetically distinct from common laboratory mice for a number of complex phenotypic characteristics and are valuable tools for genetic mapping, evolution and systematics research.
In 2019-2020, researchers at The Jackson Laboratory discovered this inbred strain contains the Trem2S148E allele - a naturally occurring variant at position 48351151-48351152 on Chr 17 (rs108080490 and rs107649577; Ensembl GRCm38.p6). This TC to GA transition results in a serine to glutamic acid substitution at amino acid 148 (S148E).
The founders were trapped in a grain warehouse, in Thonburi, Thailand by Dr. Joseph T Marshall, and mice were sent to Dr. Vernon Chapman and Dr. Frank Ruddle at Yale University, and, from there, to Dr. Eva Eicher and Dr. Thomas Roderick at The Jackson Laboratory in 1971. Dr. Eicher's colony were maintained by inbreeding to generate CAST/Ei and Dr. Roderick's colony were maintained by inbreeding to generate CASA/Rk and CASB/Rk. (Roderick, 1982; Chapman and Ruddle, 1972.)
|Allele Name||flavivirus resistance|
|Allele Synonym(s)||flavivirus resistance; Oas1bFlv-r|
|Gene Symbol and Name||Oas1b, 2'-5' oligoadenylate synthetase 1B|
|Gene Synonym(s)||2'-5' oligoadenylate synthetase 2; Mmu-L1; Oias2; Wnv; Flv; Wnv; flavivirus resistance; Flv; Oias-2; Oias2; L1; Flavivirus resistance; Oias-2; West Nile virus; OIAS; OIASI; IFI-4; E18/E16|
|Strain of Origin||various|
|General Note||The majority of mouse strains carry the susceptibility allele. Only the Det, BSVR, BRVR, CASA/RK, CAST/Ei, and PRI carry the resistance allele. The MOLD/Rk strain carried the allele for minor resistance.
Genbank ID for this allele: AF481734
|Molecular Note||This allele confers resistance to flavivirus infection.|
|Allele Name||d variant|
|Allele Type||Not Applicable|
|Allele Synonym(s)||Ahrd; d variant|
|Gene Symbol and Name||Ahr, aryl-hydrocarbon receptor|
|Gene Synonym(s)||bHLHe76; aromatic hydrocarbon responsiveness; aryl hydrocarbon hydroxylase; Ahh; dioxin receptor; In; Ah; Ahre; inflammatory reactivity; RP85|
|Strain of Origin||Not Applicable|
|General Note|| |
Strain of origin - this allele was found in DBA/2J, AKR/J, 129, SWR, RF, NZB strains
|Molecular Note||This allele encodes a 104 kDa receptor that is stabilized by molybdate and has an affinity for ligand 10-100 fold lower than that of the receptor produced by the C57BL/6J allele. PCR sequencing of cDNA revealed ten nucleotide differences between the coding sequences of the DBA/2J and C57BL/6J receptors. Five of the ten differences would cause amino acid changes. One of these, an apparent T to C transition replaces the opal termination codon in the C57BL/6J allele with an arginine codon in the DBA/2J allele. This change would extend translation of the DBA/2J mRNA by 43 amino acids, accounting for the larger size of the peptide produced by this allele (104 kDa vs 95 kDa for the C57BL/6J allele). A second T to C transition changes a leucine codon in the C57BL/6J allele to a proline codon in the DBA/2J allele, and would likely change secondary structure of the peptide and thus ligand affinity.|
|Allele Type||Not Applicable (Not Specified)|
|Allele Synonym(s)||Cox7a2ll; long|
|Gene Symbol and Name||Cox7a2l, cytochrome c oxidase subunit 7A2 like|
|Gene Synonym(s)||COX7RP; SIG-81; SIG81; silica-induced gene 81; Silg81; COX7AR; EB1|
|Strain of Origin||multiple strains|
|General Note||Querying the sequences of the Sanger Mouse Genomes Project reveals that the short allele with its 6 bp deletion exists in C57BL/6J, C57BL/10J, C57BL/6NJ, C58/J, BALB/cJ, C3H/HeH, 129S5/SvEvBrd, NZW/LacZ, and SEA/GnJ, but the long allele lacking the deletion exists in 129S1/SvImJ, A/J, AKR/J, BTBR T+ Itpr3tf/J, BUB/BnJ, C3H/HeJ, C57BR/cdJ, C57L/J, CAST/EiJ, CBA/J, DBA/1J, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LEWES/EiJ, LP/J, MOLF/EiJ, NOD/ShiLtJ, NZB/BlNJ, NZO/HlLtJ, PWK/PhJ, RF/J, SPRET/EiJ, ST/bJ, WSB/EiJ, ZALENDE/EiJ.|
|Molecular Note||This allele encodes the long isoform with 113 amino acids. It is found in 129S2/SvPasCrl, CBA/CaOlaHsd, Hsd:ICR, and NZB/OlaHsd.|
Wild-derived inbred mouse strains are maintained through sibling (sister x brother) matings; no genotyping required.
When using the CAST mouse strain in a publication, please include JAX stock #000928 in your Materials and Methods section.
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