JAX Mouse Strain Datasheet - 000802

WB.Cg-Hba^th-J/J

Stock No:: 000802 |; alpha thalassemia Jackson

Cryo Recovery

Overview

Also Known As: alpha thalassemia Jackson

These mice carry an triethylenemelamine-induced mutation at the Hba locus characterized by microcytosis, erythrocytosis, low blood hemoglobin levels, and a low body weight.

Genetic overview

Genetic Background	Generation
WB/Re

Hba^th-J

Allele Type	Gene Symbol	Gene Name
Chemically induced (other)	Hba	hemoglobin alpha chain complex

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Research Applications

Hematological Research
Developmental Biology Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery with Progeny Testing

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Details

Detailed Description

These mice carry an triethylenemelamine-induced mutation at the Hba locus characterized by microcytosis, erythrocytosis, low blood hemoglobin levels, and a low body weight.

Development

The alpha thalassemia Jackson mutation was identified in the progeny of a C57BL/6J female bred to a triethyllenemlamine treated male, but the genetic origins of that male are not defined in the literature. This mutation was backcrossed onto WB/ReJ via the continuous backcrossing of heterozygotes to an inbred at each generation. In 1988 embryos were cryopreserved from wild-type females bred to heterozygous males at generation N32.

Genetics

Hba^th-J

Allele Symbol: Hba^th-J

Allele Name	alpha thalassemia Jackson
Allele Type	Chemically induced (other)
Allele Synonym(s)	alpha^MD
Gene Symbol and Name	Hba, hemoglobin alpha chain complex
Gene Synonym(s)
Strain of Origin	C57BL/6J x Triethyllenemelamine-treated male
Chromosome	11
General Note	The mutation was induced in a male mouse by triethyllenemelamine.
Molecular Note	This mutation was shown to be a deletion that included the Hba-x, Hba-a1 and Hba-a2 genes (J:32654). The Mpg, Rhbdf1 and Phg genes are also included in the deletion region (J:29665).

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Hematological Research
- Hemoglobin Defects
  - thalassemia

Genotype: Hba^th-J related

Developmental Biology Research
- Embryonic Lethality (Homozygous)
Hematological Research
- Hemoglobin Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Hba^th-J/Hba⁺
involves: C57BL/6J

mortality/aging

postnatal lethality
- fewer than expected heterozygous mice are found at weaning (39% compared to the expected 50%)

growth/size/body region phenotype

decreased body weight

hematopoietic system phenotype

decreased hemoglobin content
- low blood hemoglobin levels

increased erythrocyte cell number
- erythrocytosis

microcytosis

Genotype: Hba^th-J/Hba⁺
B6.Cg-Hba^th-J

cellular phenotype

maternal effect
- offspring of heterozygous females, both wild-type and heterozygous offspring, have lower body weights than the offspring of wild-type mothers bred to heterozygous males

Genotype: Hba^th-J/Hba^th-J
involves: C57BL/6J

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance
- degeneration of homozygous embryos begins on E5.5 and is complete by E6.5
- no homozygous embryos can be detected by E6.5, the percentage of homozygotes in heterozygous intercross matings is reduced to only 7.7% rather than the expected 25% by E5.5, but homozygotes implant and are found at normal numbers at implantation at implantation

embryo phenotype

abnormal blastocyst morphology
- homozygous blastocysts have fewer trophectoderm cells, 78.6% of normal, and slightly fewer than normal cells of the inner cell mass and therefore have fewer total cells than normal

abnormal inner cell mass morphology
- cells of the inner cell mass of homozygous blastocysts hatched from the zona pellucida and grown in culture become necrotic and and do not remain associated with the trophoblast monolayer, as happens in wildtype blastocyst cultures

abnormal trophectoderm morphology

References

Additional - Hba^th-J related

Beckman JD; Belcher JD; Vineyard JV; Chen C; Nguyen J; Nwaneri MO; O'Sullivan MG; Gulbahce E; Hebbel RP; Vercellotti GM. 2009. Inhaled carbon monoxide reduces leukocytosis in a murine model of sickle cell disease. Am J Physiol Heart Circ Physiol 297(4):H1243-53. PubMed: 19617415 MGI: J:154228
Chang J; Lu RH; Xu SM; Meneses J; Chan K; Pedersen R; Kan YW. 1996. Inactivation of mouse alpha-globin gene by homologous recombination: mouse model of hemoglobin H disease. Blood 88(5):1846-51. PubMed: 8781443 MGI: J:35018
Fabry ME; Costantini F; Pachnis A; Suzuka SM; Bank N; Aynedjian HS; Factor SM; Nagel RL. 1992. High expression of human beta S- and alpha-globins in transgenic mice: erythrocyte abnormalities, organ damage, and the effect of hypoxia. Proc Natl Acad Sci U S A 89(24):12155-9. PubMed: 1465455 MGI: J:129537
Fabry ME; Nagel RL; Pachnis A; Suzuka SM; Costantini F. 1992. High expression of human beta S- and alpha-globins in transgenic mice: hemoglobin composition and hematological consequences. Proc Natl Acad Sci U S A 89(24):12150-4. PubMed: 1465454 MGI: J:129579
Hendrey J; Lin D; Dziadek M. 1995. Developmental analysis of the Hba(th-J) mouse mutation: effects on mouse peri-implantation development and identification of two candidate genes. Dev Biol 172(1):253-63. PubMed: 7589805 MGI: J:29665
Hendrey JA; Dziadek M. 1995. Effects on neonatal growth of the Hba(th-j) deletion on mouse chromosome 11 are not due to genomic imprinting. Growth Regul 5(3):160-3. PubMed: 7580867 MGI: J:29127
Whitney JB 3rd; Martinell J; Popp RA; Russell LB; Anderson WF. 1981. Deletions in the alpha-globin gene complex in alpha-thalassemic mice. Proc Natl Acad Sci U S A 78(12):7644-7. PubMed: 6950407 MGI: J:32654
Whitney JB 3rd; Popp RA. 1984. Animal model of human disease: thalassemia: alpha-thalassemia in laboratory mice. Am J Pathol 116(3):523-5. PubMed: 6476083 MGI: J:7581
Whitney JB 3rd; Russell ES. 1980. Linkage of genes for adult alpha-globin and embryonic alpha-like globin chains. Proc Natl Acad Sci U S A 77(2):1087-90. PubMed: 6153802 MGI: J:6282
Whitney JB; Russell ES. 1978. New mutants and biochemical variants: Alpha thalassemia Mouse News Lett 58:47-48. MGI: J:45721
Yu X; Kong Y; Dore LC; Abdulmalik O; Katein AM; Zhou S; Choi JK; Gell D; Mackay JP; Gow AJ; Weiss MJ. 2007. An erythroid chaperone that facilitates folding of alpha-globin subunits for hemoglobin synthesis. J Clin Invest 117(7):1856-65. PubMed: 17607360 MGI: J:124209

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Hba<thJ>

A molecular assay to genotype this strain is not available. We will fulfill your order by providing at least two untested males and two untested females (two pairs). The total number, sex, and genotypes will vary, although typically 8 or more mice are provided. Untested animals typically are available to ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing may be required – If recovered animals do not display a phenotype, progeny testing will be required. This testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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JAX® Mice, Products & Services Conditions of Use

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Also Known As: alpha thalassemia Jackson

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Hbath-J

Allele Symbol: Hbath-J

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Hbath-J related

Mammalian Phenotype Terms by Genotype

Genotype: Hbath-J/Hba+involves: C57BL/6J

mortality/aging

growth/size/body region phenotype

hematopoietic system phenotype

Genotype: Hbath-J/Hba+B6.Cg-Hbath-J

cellular phenotype

Genotype: Hbath-J/Hbath-Jinvolves: C57BL/6J

mortality/aging

embryo phenotype

References

Additional - Hbath-J related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Hba^th-J

Allele Symbol: Hba^th-J

Genotype: Hba^th-J related

Genotype: Hba^th-J/Hba⁺
involves: C57BL/6J

Genotype: Hba^th-J/Hba⁺
B6.Cg-Hba^th-J

Genotype: Hba^th-J/Hba^th-J
involves: C57BL/6J

Additional - Hba^th-J related