Overview

Also Known As: alpha thalassemia Jackson

These mice carry an triethylenemelamine-induced mutation at the Hba locus characterized by microcytosis, erythrocytosis, low blood hemoglobin levels, and a low body weight.

Genetic overview

Genetic Background	Generation

Hba^th-J

Allele Type	Gene Symbol	Gene Name
Chemically induced (other)	Hba	hemoglobin alpha chain complex

View Genetics

Research Applications

Hematological Research
Developmental Biology Research

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery with Progeny Testing

View Price List

Details

Detailed Description

These mice carry an triethylenemelamine-induced mutation at the Hba locus characterized by microcytosis, erythrocytosis, low blood hemoglobin levels, and a low body weight.

Development

The alpha thalassemia Jackson mutation was identified in the progeny of a C57BL/6J female bred to a triethyllenemlamine treated male, but the genetic origins of that male are not defined in the literature. This mutation was backcrossed onto WB/ReJ via the continuous backcrossing of heterozygotes to an inbred at each generation. In 1988 embryos were cryopreserved from wild-type females bred to heterozygous males at generation N32.

Genetics

Hba^th-J

Allele Symbol: Hba^th-J

Allele Name	alpha thalassemia Jackson
Allele Type	Chemically induced (other)
Allele Synonym(s)	alpha thalassemia Jackson; Hba^th-J
Gene Symbol and Name	Hba, hemoglobin alpha chain complex
Gene Synonym(s)
Strain of Origin	C57BL/6J x Triethyllenemelamine-treated male
Chromosome	11
General Note	The mutation was induced in a male mouse by triethyllenemelamine.
Molecular Note	This mutation was shown to be a deletion that included the Hba-x, Hba-a1 and Hba-a2 genes (J:32654). The Mpg, Rhbdf1 and Phg genes are also included in the deletion region (J:29665).

Disease/Phenotype

Disease Terms

Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.

alpha thalassemia

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Hematological Research
- Hemoglobin Defects
  - thalassemia

Genotype: Hba^th-J related

Developmental Biology Research
- Embryonic Lethality (Homozygous)
Hematological Research
- Hemoglobin Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Hba^th-J/Hba⁺
involves: C57BL/6J

growth/size/body region phenotype

decreased body weight

hematopoietic system phenotype

decreased hemoglobin content
- low blood hemoglobin levels

increased erythrocyte cell number
- erythrocytosis

microcytosis

mortality/aging

postnatal lethality
- fewer than expected heterozygous mice are found at weaning (39% compared to the expected 50%)

Genotype: Hba^th-J/Hba^th-J
involves: C57BL/6J

embryo phenotype

abnormal blastocyst morphology
- homozygous blastocysts have fewer trophectoderm cells, 78.6% of normal, and slightly fewer than normal cells of the inner cell mass and therefore have fewer total cells than normal

abnormal inner cell mass morphology
- cells of the inner cell mass of homozygous blastocysts hatched from the zona pellucida and grown in culture become necrotic and and do not remain associated with the trophoblast monolayer, as happens in wildtype blastocyst cultures

abnormal trophectoderm morphology

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance
- at implantation
- degeneration of homozygous embryos begins on E5.5 and is complete by E6.5
- no homozygous embryos can be detected by E6.5, the percentage of homozygotes in heterozygous intercross matings is reduced to only 7.7% rather than the expected 25% by E5.5, but homozygotes implant and are found at normal numbers at implantation

Genotype: Hba^th-J/Hba⁺
B6.Cg-Hba

cellular phenotype

maternal effect
- offspring of heterozygous females, both wild-type and heterozygous offspring, have lower body weights than the offspring of wild-type mothers bred to heterozygous males

References

Additional - Hba^th-J related

Technical Support

Contact Technical Support

Genotyping Protocols
- Genotyping resources and troubleshooting
Citation
When using the alpha thalassemia Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000802 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Hba<thJ>

A molecular assay to genotype this strain is not available. We will fulfill your order by providing at least two untested males and two untested females (two pairs). The total number, sex, and genotypes will vary, although typically 8 or more mice are provided. Untested animals typically are available to ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing may be required – If recovered animals do not display a phenotype, progeny testing will be required. This testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: alpha thalassemia Jackson

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Hbath-J

Allele Symbol: Hbath-J

Disease Terms

Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Hbath-J related

Mammalian Phenotype Terms by Genotype

Genotype: Hbath-J/Hba+involves: C57BL/6J

growth/size/body region phenotype

hematopoietic system phenotype

mortality/aging

Genotype: Hbath-J/Hbath-Jinvolves: C57BL/6J

embryo phenotype

mortality/aging

Genotype: Hbath-J/Hba+B6.Cg-Hba

cellular phenotype

References

Additional - Hbath-J related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Hba^th-J

Allele Symbol: Hba^th-J

Genotype: Hba^th-J related

Genotype: Hba^th-J/Hba⁺
involves: C57BL/6J

Genotype: Hba^th-J/Hba^th-J
involves: C57BL/6J

Genotype: Hba^th-J/Hba⁺
B6.Cg-Hba

Additional - Hba^th-J related