Similar to the original bouncy allele (bc), mice homozygous for bouncy 3J (bc3J) exhibit moderate tremors, an impaired (bouncy) gait, male infertility and small body size. This mutant mouse strain may be useful in neurological studies of motor coordination.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Kcnn2 | potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 |
Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Clcc1 | chloride channel CLIC-like 1 |
Similar to the original bouncy allele (bc), mice homozygous for bouncy 3J (bc3J) exhibit moderate tremors, an impaired (bouncy) gait, male infertility and small body size. Some homozygous mice die before weaning; mice that survive have a normal lifespan. This mutant mouse strain may be useful in neurological studies of motor coordination. The Clcc1m1J spontaneous mutation, which causes increased sensitivity to endoplasmic reticulum stress in the cerebellum, was identified in C3H/HeSnJ (Jia et al., 2015) and has been found homozygous in all C3H/HeSn strains assessed at The Jackson Laboratory, including this mutant subline, which is homozygous for Clcc1m1J.
Bouncy 3 Jackson (bc3J) arose spontaneously in strain C3H/HeSn in the Foundation Stocks at The Jackson Laboratory in 1980 when that inbred was at F90. The stock was maintained by sibling matings of homozygous (bc3J/bc3J) females to heterozygous males or by mating heterozygotes. Embryos were generated for cryopreservation in 1981 by intercrossing heterozygotes at generation F12.
Allele Name | bouncy 3 Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Kcnn2, potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 |
Gene Synonym(s) | |
Strain of Origin | C3H/HeSn |
Chromosome | 18 |
Molecular Note | A G-to-A point mutation at coding nucleotide 599/1394/1424 of the transcript (NM_080465/ENSMUST00000066890/NM_001312905) is predicted to result in premature truncation at tryptophan 200/465/475 of the encoded protein (NP_536713:p.W200*, ENSMUSP00000067884:p.W465*, NP_001299834:p.W475*). |
Allele Name | mutation 1, Jackson |
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Allele Type | Spontaneous |
Allele Synonym(s) | nm2453; sprawler |
Gene Symbol and Name | Clcc1, chloride channel CLIC-like 1 |
Gene Synonym(s) | |
Strain of Origin | C3H/HeSnJ |
Chromosome | 3 |
Molecular Note | The mutation is an intracisternal A-particle (IAP) retrotransposon insertion into a C3H/HeSnJ genomic region. The IAP is inserted into intron 2 of the gene and disrupts the normal splicing of the mRNA transcribed from this gene. Resulting in-frame stop codons from the insertion of IAP sequences resulted in severely reduced protein levels in mutant tissues. The retrotransposition of this IAP is a recent event occurring during, or after, the establishment of the C3H/HeSn substrain. |
The colony is maintained by mating homozygous females to heterozygous males or by mating heterozygote x heterozygote. Homozygous males are sterile. Homozygous mutants must be at least 4 weeks of age to be shipped.
When using the C3H/HeSn-bc3J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000775 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Homozygous for bc<3J>, 1 pair minimum |
Frozen Mouse Embryo | C3H/HeSn-bc<3J>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C3H/HeSn-bc<3J>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C3H/HeSn-bc<3J>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C3H/HeSn-bc<3J>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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