DW.C3-Mlph⁺ Pou1f1⁺/J

Stock number: 000681
Product name: dwarf
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Mouse/Human Gene Homologs

Description

These mice carry a spontaneous mutation at the Pou1f1 locus characterized by severe dwarfing, sterility, and hypothyroidism. The strain also carries the coat color mutation, leaden, as an aid for identifying genotypes.

Detailed description

This DW/J congenic strain is wildtype for both the leaden (Mlph⁺) and dwarf (Pou1f1⁺) mutations and thus serves as a control strain for DW/J Mlph^ln Pou1f1^dw/J (Stock No. 000643).

(Pou1f1 was formerly Pit1)

Development

This control strain for DW/J was made to provide compatible hosts for ovarian transplantation. In 1966 the DW/J strain was sent to The Jackson Laboratory Animal Resources Mutant Stocks department. At this time the stock was maintained by tested sibling matings. In order to use ovarian transplants a compatible host and color coded strain was started by crossing a DW/J mouse to a C3H/HeJ .The offspring of this cross, a non leaden and non dwarf mouse, was crossed back to strain DW/J each generation to N10. At generation N11 the pairs were tested by breeding to be non-carriers of leaden and dwarf making this control line Mlph⁺ and Pit1⁺. It was cryopreserved in 1992 by mating the homozygous wild type males and females at N11 F20.

Allele

Symbol: Mlph⁺
Name: wild type
MGI accession ID: MGI:2441644
Generation method: Not Applicable
Marker symbol: Mlph
Marker name: melanophilin
Chromosome: 1
Strain of origin: Not Applicable

Allele

Symbol: Pou1f1⁺
Name: wild type
MGI accession ID: MGI:2431704
Generation method: Not Applicable
Marker symbol: Pou1f1
Marker name: POU domain, class 1, transcription factor 1
Chromosome: 16
Strain of origin: Not Applicable