T(8;16)17H was found in the daughter of CBA/H female x X-irradiated (C3H x 101)F1 male. This daughter was bred to a C57BL/H male (Searle, AG, 1964). This translocation was maintained at The Jackson Laboratory by breeding to B6C3HF1 at each generation prior to crossing it to Stock No. 000510, C3H/HeJ-Pou1f1dw-J/J.
|Gene Symbol and Name||a, nonagouti|
|Strain of Origin||old mutant of the mouse fancy|
|General Note||Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039|
|Molecular Note||Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.|