B6-Gpi1^b x B6CBCa A^w-J/A-T(7;15)9H Gpi1^a/J

Stock number: 000600
Product name: T9H
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Development

The T(7;15)9H translocation was induced prior to 1964 in a (C3H/HeH x 101/H)F1 male by exposure to 600R X-rays at 6 weeks of age and again at 14 weeks of age. Twelve weeks after the last exposure the male was bred to a CBA/H female and the female offspring of this cross were bred to C57BL/H males. Subsequent breeding is not known until this translocation was imported into the laboratory of Dr. Eva Eicher at The Jackson Laboratory in the mid-1970?s. It was bred to (C57BL/6J-A^w-J/J x CBA/CaGnLeJ)F1 mice for 7 generations. The Gpi1^a allele co-segregates with the translocation and C57BL/6J mice have the Gpi1^b allele. Thus, Gpi1^a was used as a marker for the likely presence of the translocation in breeder selection. This strain was later intercrossed to homozygosity and maintained by sibling inbreeding translocation homozygotes. In 1983 homozygous males, then at generation N7F8, were bred t o C57BL/6J females to generate embryos for cryopreservation.

Allele

Symbol: A^w-J
Name: white bellied agouti Jackson
MGI accession ID: MGI:1855946
Generation method: Spontaneous
Marker symbol: a
Marker name: nonagouti
Chromosome: 2
Strain of origin: C57BL/6J

Allele

Symbol: Gpi1^b
Name: b variant
MGI accession ID: MGI:2177270
Generation method: Not Applicable
Marker symbol: Gpi1
Marker name: glucose phosphate isomerase 1
Chromosome: 7
Strain of origin: Not Applicable
Molecular note: This allele determines a faster migrating variant of the encoded enzyme and occurs in the wild and in strains BUB/Bn, CBA/J, CBA/CaJ, C3H/HeH, C3Heb/FeJ, C57BL/6J, C57BL/6J-ob, SEA/GJ and SWR/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1^a. Two differences were silent, while a third difference at nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted.

Allele

Symbol: Gpi1^a
Name: a variant
MGI accession ID: MGI:2177271
Generation method: Not Applicable
Marker symbol: Gpi1
Marker name: glucose phosphate isomerase 1
Chromosome: 7
Strain of origin: Not Applicable
Molecular note: This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains 101/H, A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1^b. Two differences are silent, while a third difference at position nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted.

Allele

MGI accession ID: MGI:103967
Marker symbol: T(7;15)9H
Marker name: reciprocal translocation, Chr 7 and 15, Harwell 9
Chromosome: 7

Allele

MGI accession ID: MGI:103968
Marker symbol: T(7;15)9H
Marker name: reciprocal translocation, Chr 7 and 15, Harwell 9
Chromosome: 15