Overview

Also Known As:beige 2 Jackson

These mice carry a spontaneous mutation at the Lyst locus characterized by immunodeficiency and reduced pigmentation of the coat with progressive darkening of the eyes.

Genetic overview

Genetic Background	Generation

Lyst^bg-2J

Allele Type	Gene Symbol	Gene Name
Spontaneous	Lyst	lysosomal trafficking regulator

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Research Applications

Metabolism Research
Cardiovascular Research
Dermatology Research
Hematological Research
Immunology, Inflammation and Autoimmunity Research
Internal/Organ Research
Mouse/Human Gene Homologs
Research Tools

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Important Note

This strain is homozygous for the retinal degeneration allele Pde6b^rd1.

Detailed Description

Mice homozygous for the beige 2J spontaneous mutation (Lyst^bg-2J) display characteristics very similar to the original beige mutation (Lyst^bg). The mouse phenotype closely resembles Chediak-Higashi syndrome in man and similar conditions in mink and cattle. Abnormal giant lysosomal granules occur in all tissues with granule-containing cells, including granulocytes, lymphocytes, cells of the liver, kidney, central nervous system, pancreas, and thyroid, and the ducts of most glands; in type II pneumocytes; in mast cells; and in retinal pigment epithelium. Granulocytes from beige homozygous mutant mice show defective chemotaxis and reduced bactericidal activity. Beige mice are more susceptible than controls to pneumonitis and to various viral, bacterial, and parasitic infections. Natural killer (NK) cells from beige mice exhibit decreased endogenous cytotoxic activity. Beige mice also have a defective cytotoxic T-cell and cytotoxic antibody response to allogeneic tumor cells. Syngeneic tumor cells grow better in beige mice than in littermate controls, an effect thought to be due to the deficiency of NK cells. Beige mice have platelet storage pool deficiency, leading to a prolonged bleeding time. The immunodeficiency of beige mutant mice has been used, especially in combination with the scid mutation (Prkdc^scid), in tissue graft and disease studies.

Control Suggestions

Heterozygote from the colony
000659 C3H/HeJ

Additional Information

Considerations for Choosing Controls

Genetics

Lyst^bg-2J

Allele Symbol: Lyst^bg-2J

Allele Name	beige 2 Jackson
Allele Type	Spontaneous
Allele Synonym(s)
Gene Symbol and Name	Lyst, lysosomal trafficking regulator
Gene Synonym(s)
Strain of Origin	Yellow stock
Chromosome	13
Molecular Note	This allele was identified by a noncomplementation test with Lyst^bg. Quantitative reverse transcription showed the molecular fault in the Lyst^bg-2J mutation may be an intronic retrotransposition event causing a five-fold decrease in transcription and mRNA instability.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Lyst^bg-2J related

Metabolism Research
Cardiovascular Research
- Atherosclerosis
Dermatology Research
- Color and White Spotting Defects
Hematological Research
- Immunological Defects
- Platelet Defects
  - platelet storage pool deficiency
Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Immunodeficiency
- Intracellular Signaling Molecules
Internal/Organ Research
- Kidney Defects
  - lysosomal enzyme abnormalities
Mouse/Human Gene Homologs
- Chediak-Higashi syndrome
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - NK Cell Deficiency

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Lyst^bg-2J/Lyst^bg-2J
Not Specified

craniofacial phenotype

decreased ear pigmentation
- pigmentation is reduced

vision/eye phenotype

abnormal eye pigmentation
- eye color is light at birth, varying from ruby to almost black in adults

decreased eye pigmentation

growth/size/body region phenotype

decreased ear pigmentation
- pigmentation is reduced

hearing/vestibular/ear phenotype

decreased ear pigmentation
- pigmentation is reduced

integument phenotype

decreased ear pigmentation
- pigmentation is reduced

decreased tail pigmentation
- pigmentation is reduced

diluted coat color
- coat color is lighter than that of normal wild-type mice of the same background

limbs/digits/tail phenotype

decreased tail pigmentation
- pigmentation is reduced

pigmentation phenotype

abnormal eye pigmentation
- eye color is light at birth, varying from ruby to almost black in adults

decreased ear pigmentation
- pigmentation is reduced

decreased eye pigmentation

decreased tail pigmentation
- pigmentation is reduced

diluted coat color
- coat color is lighter than that of normal wild-type mice of the same background

The following phenotype relates to a compound genotype created using this strain

Genotype: Lyst^bg-2J/Lyst^bg-2J
C3.Cg-Lyst/J

behavior/neurological phenotype

abnormal gait
- an impaired gait is obvious by 17 months of age

impaired balance
- obvious by 17 months of age

impaired swimming
- mice lose ability to swim by 17 months of age

paraparesis
- motor weakness of the hind legs is noticeable by 17 months of age

tremors
- a fine sustained tremor is observed by 17 months of age

nervous system phenotype

decreased Purkinje cell number
- mice in their second year of life and without obvious abnormal neurological behavior have a significant reduction in number of cells
- there is a complete or nearly complete loss of cells in mice showing neurological symptoms by 17 months of age

immune system phenotype

altered susceptibility to infection

decreased susceptibility to fungal infection
- Background Sensitivity - mice are less susceptible to experimental cryptococcosis infection than mice homozygous for the Lyst^bg-J allele on C57BL/6

neoplasm

altered metastatic potential

decreased metastatic potential
- mutant mice had a significantly higher retention of the introduced tumor variant, CIRAS 1, than normal mice after an exercise regime or sedentary activity
- mutant mice randomly assigned 9 week treadmill training had lower CIRAS 1 tumor cell retention (44 +/- 3%) than mice displaying sedentary behavior (53 +/- 3%); there was no difference seen for the more aggressive tumor variant CIRAS 3

References

Additional References

Gallin JI; Bujak JS; Patten E; Wolff SM. 1974. Granulocyte function in the Chediak-Higashi syndrome of mice. Blood 43(2):201-6PubMed: 4589319 MGI: J:5405
Perou CM; Moore KJ; Nagle DL; Misumi DJ; Woolf EA; McGrail SH; Holmgren L; Brody TH; Dussault BJ Jr; Monroe CA; Duyk GM; Pryor RJ; Li L; Justice MJ; Kaplan J. 1996. Identification of the murine beige gene by YAC complementation and positional cloning. Nat Genet 13(3):303-8PubMed: 8673129 MGI: J:33734
Roder JC. 1979. The beige mutation in the mouse. I. A stem cell predetermined impairment in natural killer cell function. J Immunol 123(5):2168-73PubMed: 489978 MGI: J:6213
Schiller NK; Black AS; Bradshaw GP; Bonnet DJ; Curtiss LK. 2004. Participation of macrophages in atherosclerotic lesion morphology in LDLr-/- mice. J Lipid Res 45(8):1398-409PubMed: 15175354 MGI: J:91367

Additional - Lyst^bg-2J related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- light agouti, affected
  Related Genotype: A/A Lyst^bg-2J/Lyst^bg-2J
  
  agouti, unaffected
  Related Genotype: A/A Lyst^bg-2J/+
Citation
When using the beige 2 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #000509 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Homozygous for Lyst<bg-2J>

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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Also Known As:beige 2 Jackson

Genetic overview

Research Applications

Base Price

Important Note

Detailed Description

Control Suggestions

Additional Information

Lystbg-2J

Allele Symbol: Lystbg-2J

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Lystbg-2J related

Mammalian Phenotype Terms by Genotype

Genotype: Lystbg-2J/Lystbg-2JNot Specified

craniofacial phenotype

vision/eye phenotype

growth/size/body region phenotype

hearing/vestibular/ear phenotype

integument phenotype

limbs/digits/tail phenotype

pigmentation phenotype

Genotype: Lystbg-2J/Lystbg-2JC3.Cg-Lyst/J

behavior/neurological phenotype

nervous system phenotype

immune system phenotype

neoplasm

References

Additional References

Additional - Lystbg-2J related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Lyst^bg-2J

Allele Symbol: Lyst^bg-2J

Genotype: Lyst^bg-2J related

Genotype: Lyst^bg-2J/Lyst^bg-2J
Not Specified

Genotype: Lyst^bg-2J/Lyst^bg-2J
C3.Cg-Lyst/J

Additional - Lyst^bg-2J related