B10.Cg-Tgm3^we-Bkr/CyJ

Stock number: 000475
Product name: wellhaarig-Bunker
Research areas: Dermatology Research, Developmental Biology Research

Description

This spontaneous single amino acid substitution causes homozygotes to have permanently curly vibrissae and curly hair early in life, which becomes more normal with age.

Detailed description

Homozygotes present with curly vibrissae as early as 2 to 3 days of age, and have curly vibrissae throughout their lives. When the coat grows in at approximately 2 weeks of age it has a noticeably wavy texture, which becomes more normal as the mice age. The hairs themselves are thinner than normal, in addition to their abnormal waviness. Homozygotes are viable and fertile.

Development

The wellhaarig-Bunker allele arose spontaneously in 1965 in the Snell congenic strain then name B10.129P-H46^b H47^b/(21M)Sn, which was subsequently updated to B10.129P-H46^b Selenos^b/(21M)Sn (Stock No. 000414). The parental congenic was at generation NE11 when this mutation arose and one backcross was done to C57BL/10Sn to remove the pink-eyed dilution and H-4 alleles. This mutant subline was subsequently maintained by sibling intercrossing and in 1984 embryos were generated for cryopreservation from homozygous males and females at generation NE11+1+F42.

Allele

Symbol: Tgm3^we-Bkr
Name: wellhaarig Bunker
MGI accession ID: MGI:1929708
Generation method: Spontaneous
Attributes: Not Specified
Marker symbol: Tgm3
Marker name: transglutaminase 3, E polypeptide
Chromosome: 2
Strain of origin: B10.129P-Il4i1^H46-b Emp3^b Oca2^p Selenos^H47-b/(21M)Sn
Molecular note: A spontaneous C-to-T point mutation (c.896C>T) in exon 7s substitute a highly-conserved polar serine with a non-polar leucine residue at position 299 (p.S299L).