This spontaneous single amino acid substitution causes homozygotes to have permanently curly vibrissae and curly hair early in life, which becomes more normal with age.Read More +
Homozygotes present with curly vibrissae as early as 2 to 3 days of age, and have curly vibrissae throughout their lives. When the coat grows in at approximately 2 weeks of age it has a noticeably wavy texture, which becomes more normal as the mice age. The hairs themselves are thinner than normal, in addition to their abnormal waviness. Homozygotes are viable and fertile.
The wellhaarig-Bunker allele arose spontaneously in 1965 in the Snell congenic strain then name B10.129P-H46b H47b/(21M)Sn, which was subsequently updated to B10.129P-H46b Selenosb/(21M)Sn (Stock No. 000414). The parental congenic was at generation NE11 when this mutation arose and one backcross was done to C57BL/10Sn to remove the pink-eyed dilution and H-4 alleles. This mutant subline was subsequently maintained by sibling intercrossing and in 1984 embryos were generated for cryopreservation from homozygous males and females at generation NE11+1+F42.
|Allele Name||wellhaarig Bunker|
|Allele Type||Spontaneous (Not Specified)|
|Gene Symbol and Name||Tgm3, transglutaminase 3, E polypeptide|
|Strain of Origin||B10.129P-Il4i1H46-b Emp3b Oca2p SelenosH47-b/(21M)Sn|
|Molecular Note||A spontaneous C-to-T point mutation (c.896C>T) in exon 7s substitute a highly-conserved polar serine with a non-polar leucine residue at position 299 (p.S299L).|