B10.D2-Hc⁰ H2^d H2-T18^c/o2SnJ

Stock number: 000460
Product name: B10.D2/oSn
Research areas: Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This congenic strain carries the H2^d haplotype along with H2-T18^c and Hc⁰ from the DBA/2 inbred strain.

Detailed description

This congenic strain carries the H2^d haplotype from DBA/2J following six generations of backcrossing to C57BL/10Sn. This strain still carries the Hc⁰ allele from DBA/2J, making them serum C5 deficient. Mice have increased susceptibility to certain pathogens and impaired chemotactic responses of neutrophils. Allograft rejection is prolonged. The following inbred strains are also homozygous for the Hc⁰ allele: A/HeJ (Stock No. 000645), AKR/J (Stock No. 000648), DBA/2J (Stock No. 000671), NOD/LtJ (Stock No. 001976), NZB/BlNJ (Stock No. 000684), and SWR/J (Stock No. 000689).

Development

This is one of the “Congenic Resistant” strains of mice developed by George D. Snell in his studies of histocompatibility. [Snell, G.D. 1978. In Origins of Inbred Mice (H.C. Morse III, ed.). Academic Press, New York.]

The strain was backcrossed the equivalent of 12 generations to C57BL/10Sn before being maintained by sib matings. It was cryopreserved in 1994. It is distinguished from 000461 B10.D2-Hc⁰ H2^d H2-T18^c/oSnJ by the number of backcross generations.

Allele

Symbol: Hc⁰
Name: deficient
MGI accession ID: MGI:3027637
Generation method: Spontaneous
Synonyms: C5-; C5-d; C5-def; C5-deficient; Hc^Hfib2; hc^o
Marker symbol: Hc
Marker name: hemolytic complement
Marker synonyms: C5; C5a; C5b; C5D; CPAMD4; ECLZB; He; Hfib2; RGD1561905
Chromosome: 2
Strain of origin: multiple strains
Molecular note: A 2 base "TA" deletion at positions 62 and 63 of an 83 base pair exon near the 5' end of the gene is found in the following mouse strains: A/HeJ, A/J, AKR/J, DBA/2J, I/LnJ, KK/HlJ, MOLF/EiJ, NZB/B1NJ, RF/J, ST/bJ SWR/J, B10.D2/oSnJ. The consequence of this deletion is the creation of a stop codon starting four bases after the deletion. A truncated product of 216 amino acids is predicted as a result although contradictory reports exist that a larger pro-C5 protein may be synthesized. Nevertheless, macrophages from mouse strains carrying this allele do not secrete complement 5.
General note:

This is an allele characteristic of various inbred mouse strains including the following: A/HeJ, A/J, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The A/J allele contains a 2 bp deletion resulting in deficient Hc mRNA and protein production and is associated with susceptibility to allergen-induced bronchial hyperresponsiveness. (J:108211)

Allele

Symbol: H2^d
Name: d variant
MGI accession ID: MGI:3579320
Generation method: Spontaneous
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Marker synonyms: H-2; MHC-II
Chromosome: 17
Strain of origin: various
General note: The d variant has been observed in the following strains: DBA/2, DBA/2J BALB/c, BALB/cByJ, BALB/cJ, C57BLKS, NZB.

Allele

Symbol: H2-T18^c
Name: c variant
MGI accession ID: MGI:4820509
Generation method: Not Applicable
Synonyms: Tla^c
Marker symbol: H2-T18
Marker name: histocompatibility 2, T region locus 18
Marker synonyms: H-2T18; TL Ag; Tla
Chromosome: 17
Strain of origin: Not Applicable
Molecular note: The allele H2-T18^c determines presence of specificities TL.2, 7 and occurs in strains BALB/c, DBA/2, 129/J, and others.