Mice homozygous for the jaundiced mutation are very pale but not jaundiced at birth, but develop severe jaundice within hours of being born. They have severe microcytic hemolytic anemia, and most die by 4 days of age, even on a mixed background, but a single blood transfusion in the first days of life can foster survival of homozygotes. On a mixed C57BL/6J x WB/Re background transfused homozygotes generally survive to adulthood and are reported to have a mean life expectancy of 3.7 months. The hemolytic anemia phenotype of these adults includes decreased hematocrit, very low red blood cell count, reticulocytosis, microcytosis, bilirubinemia, extensive iron accumulation in the kidney, elevated blood urea nitrogen, hydronephrosis, hepatomegaly, splenomegaly of predominantly red pulp, and cardiomegaly, but less severe thrombosis than is found in mice homozygous for the mutation spherocytosis (Spna1sph). The erythrocytes are extremely fragile and have a very short lifespan and there is extramedullary hematopoiesis. Heterozygotes have a mild, well compensated anemia and the erythrocytes have a higher concentration of protoporphyrin, increased osmotic fragility, and a slightly reduced lifespan.
The jaundiced mutation, first described in 1959, arose spontaneously at The Jackson Laboratory in a 129/Sv inbred strain that was homozygous for pink-eyed dilution and segregating for albino and chinchilla. Jaundiced was backcrossed separately onto the C57BL/6J and WB/Re backgrounds by Seldon Bernstein and each congenic strain reached generation N27 in 1968. This strain reached generation N49 in 1978 and was later passed to Dr. Jane Barker. In 2010 sperm was cryopreserved from heterozygous males at generation N81.
|Allele Synonym(s)||jaundiced; Sptbja|
|Gene Symbol and Name||Sptb, spectrin beta, erythrocytic|
|Gene Synonym(s)||D330027P03Rik; D330027P03Rik; brain erythroid spectrin (235E); Spnb-1; Spnb1; LOC383567; HSPTB1; HS2; ja; Spnb1; jaundiced; gene model 1301, (NCBI); Spnb-1; spectrin R; SPH2; expressed sequence AI842465; EL3; Gm1301; RIKEN cDNA D330027P03 gene; AI842465; spectrin beta 1|
|Strain of Origin||129/Sv|
|Molecular Note||A C-to-T transition mutation is present in the mutant transcript and produces a premature stop codon from an arginine codon in mRNA encoding repeat 9 of beta-spectrin at amino acid position 1160.|
|Heterozygous or wildtype for Spnb1<ja>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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