STOCK a/a Myo5a^d fd/+ +/J

Stock number: 000286
Research areas: Dermatology Research, Mouse/Human Gene Homologs

Development

Fur deficient (fd) arose spontaneously in DBA/2J in 1966 at The Jackson Laboratory. It was outcrossed to C57BL/6J once and the F1 was backcrossed to a DBA2/J-fd homozygote. It was then sibling mated using a Myo5a^d fd/Myo5a^d fd male and a Myo5a^d fd/+ + female. Close linkage was found between Myo5a^d and fd and the stock was maintained by sibling matings keeping the two loci in coupling phase. It was cryopreserved in 1991 by mating Myo5a^d fd/Myo5a^d fd or Myo5a^d fd/+ + males at F47 to C57BL/6J females.

Allele

Symbol: fd
Name: fur deficient
MGI accession ID: MGI:1856867
Generation method: Spontaneous
Marker symbol: fd
Marker name: fur deficient
Chromosome: 9
Strain of origin: DBA/2J

Allele

Symbol: a
Name: nonagouti
MGI accession ID: MGI:1855937
Generation method: Spontaneous
Marker symbol: a
Marker name: nonagouti
Chromosome: 2
Strain of origin: old mutant of the mouse fancy
Molecular note: Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for a^t-2Gso and A^w-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.
General note: Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039

Allele

Symbol: Myo5a^d
Name: dilute
MGI accession ID: MGI:1856004
Generation method: Spontaneous
Marker symbol: Myo5a
Marker name: myosin VA
Chromosome: 9
Strain of origin: old mutant of the mouse fancy
Molecular note: This mutation is the result of the integration of ecotropic murine leukemia virus Emv-3 into a noncoding region of the Myo5a^d gene. Reversions of Myo5a^d to wild-type are caused by excision of the virus leaving exactly one long terminal repeat in place.