Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Myo5a | myosin VA |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | fd | fur deficient |
Fur deficient (fd) arose spontaneously in DBA/2J in 1966 at The Jackson Laboratory. It was outcrossed to C57BL/6J once and the F1 was backcrossed to a DBA2/J-fd homozygote. It was then sibling mated using a Myo5ad fd/Myo5ad fd male and a Myo5ad fd/+ + female. Close linkage was found between Myo5ad and fd and the stock was maintained by sibling matings keeping the two loci in coupling phase. It was cryopreserved in 1991 by mating Myo5ad fd/Myo5ad fd or Myo5ad fd/+ + males at F47 to C57BL/6J females.
Allele Name | nonagouti |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 2 |
General Note | Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039 |
Molecular Note | Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type. |
Allele Name | dilute |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | blue dilution; d; dv; Maltese dilution |
Gene Symbol and Name | Myo5a, myosin VA |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 9 |
Molecular Note | This mutation is the result of the integration of ecotropic murine leukemia virus Emv-3 into a noncoding region of the Myo5ad gene. Reversions of Myo5ad to wild-type are caused by excision of the virus leaving exactly one long terminal repeat in place. |
Myo5d and fd are linked on chromosome 9 and maintained in coupling in this strain.
When maintaining a live colony, mice can be bred as heterozygote x homozygote or homozygote x heterozygote for Myo5ad; and heterozygote x homozygote or homozygote x heterozygote for fd.
When using the STOCK a/a Myo5ad fd/+ +/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000286 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for a, Heterozygous for Myo5a<d>, Heterozygous for fd |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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