CWD/LeJ

Stock number: 000284
Product name: curly whiskers dilute

Strain synonyms: CWD/Agl, CWD/LeAgl
Research areas: Dermatology Research, Mouse/Human Gene Homologs

Description

This inbred mutant strain carries the curly whiskers mutation (Hephl1^cw) characterized by strongly curled whiskers in homozygotes, but coat hair is not waved.

Development

The curly whiskers mutation (cw) arose spontaneously in 1958 in a CBA strain at the Chester Beatty Research Institute in London and was imported to The Jackson Laboratory. The imported genotype was cw + +/cw se tk, a^t/a b/+ c^ch/+. It was maintained by Dr. M.C. Green and was outcrossed once to a B6C3Fe-a/a F1 and then once to a DBA. It was within-stock mated for 3 generations and the cw d/+ d x cw d/cw d genotype was selected for inbreeding. It was brother x sister bred and reached generation F91 in 1995. It was cryopreserved in 1989 by mating cw d/+ d x cw d/cw d, but was subsequently thawed and re-frozen from homozygous females bred with homozygous males so the bankstock now available is homozygous cw d/cw d.

Allele

Symbol: a
Name: nonagouti
MGI accession ID: MGI:1855937
Generation method: Spontaneous
Marker symbol: a
Marker name: nonagouti
Chromosome: 2
Strain of origin: old mutant of the mouse fancy
Molecular note: Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for a^t-2Gso and A^w-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.
General note: Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039

Allele

Symbol: Myo5a^d
Name: dilute
MGI accession ID: MGI:1856004
Generation method: Spontaneous
Marker symbol: Myo5a
Marker name: myosin VA
Chromosome: 9
Strain of origin: old mutant of the mouse fancy
Molecular note: This mutation is the result of the integration of ecotropic murine leukemia virus Emv-3 into a noncoding region of the Myo5a^d gene. Reversions of Myo5a^d to wild-type are caused by excision of the virus leaving exactly one long terminal repeat in place.