This inbred mutant strain carries the curly whiskers mutation (Hephl1cw) characterized by strongly curled whiskers in homozygotes, but coat hair is not waved.
Read More +This cryopreserved bankstock is homozygous for nonagouti, Myo5ad and Hephl1cw-2J.
The curly whiskers mutation (cw) arose spontaneously in 1958 in a CBA strain at the Chester Beatty Research Institute in London and was imported to The Jackson Laboratory. The imported genotype was cw + +/cw se tk, at/a b/+ cch/+. It was maintained by Dr. M.C. Green and was outcrossed once to a B6C3Fe-a/a F1 and then once to a DBA. It was within-stock mated for 3 generations and the cw d/+ d x cw d/cw d genotype was selected for inbreeding. It was brother x sister bred and reached generation F91 in 1995. It was cryopreserved in 1989 by mating cw d/+ d x cw d/cw d, but was subsequently thawed and re-frozen from homozygous females bred with homozygous males so the bankstock now available is homozygous cw d/cw d.
Allele Name | nonagouti |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 2 |
General Note | Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039 |
Molecular Note | Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type. |
Allele Name | dilute |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | blue dilution; d; dv; Maltese dilution |
Gene Symbol and Name | Myo5a, myosin VA |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 9 |
Molecular Note | This mutation is the result of the integration of ecotropic murine leukemia virus Emv-3 into a noncoding region of the Myo5ad gene. Reversions of Myo5ad to wild-type are caused by excision of the virus leaving exactly one long terminal repeat in place. |
Allele Name | curly whiskers |
---|---|
Allele Type | Spontaneous (Hypomorph) |
Allele Synonym(s) | cw |
Gene Symbol and Name | Hephl1, hephaestin-like 1 |
Gene Synonym(s) | |
Strain of Origin | CBA/Cbi |
Chromosome | 9 |
Molecular Note | This spontaneous A to G point substitution at Chr9:15076948 (GRCm38.p6) in the splice acceptor 2 base pairs upstream of exon 11 results in 2 variant transcripts assessed as cDNA from heterozygous skin, of which the 581 bp variant has an early stop codon and is likely to be eliminated by nonsense-mediated decay, and the 397 bp variant is predicted to be stable but is found in lower abundance than the 606 bp wild type transcript. |
When using the curly whiskers dilute
mouse strain in a publication, please include JAX stock #000284 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Inbred, 1 pair minimum will be supplied |
Frozen Mouse Embryo | CWD/LeJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | CWD/LeJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | CWD/LeJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | CWD/LeJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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