LT.CAST-A/J

Stock number: 000283

Development

LT/SvEi is homozygous for the nonagouti (a) mutation. The Agouti allele (A) was backcrossed from CAST/Ei onto LT/SvEi for 10 generations before intercrossing. In 1987 N10F28 homozygotes were bred together to generate embryos for cryopreservation. (Eppig and Eicher, 1983.)

Allele

Symbol: A
Name: wild-type agouti
MGI accession ID: MGI:1856464
Generation method: Spontaneous
Synonyms: dark-bellied agouti
Marker symbol: a
Marker name: nonagouti
Marker synonyms: agouti; agouti signal protein; AGSW; AGTI; AGTIL; As; ASP; SHEP9
Chromosome: 2
Strain of origin: various
Molecular note: This allele, often referred to as wild-type, comprises a novel 131 amino acid protein encoded in a gene comprising four exons, three coding, spanning 18kb. Unique changes in this gene account for all other alleles that have been molecularly characterized. The expression of this allele is almost always dominant to other alleles of this gene.
General note: The A allele is usually regarded as a wild-type allele. For example,the C3H and CBA mouse sublines are homozygous for agouti. Hairs are black with a subapical yellow band. This black-yellow-black pattern is referred to as agouti. The general appearance is yellowish brown, slightly lighter on the belly than on the back.