This strain is homozygous for nonagouti (a).
Mice homozygous for the recessive buff (Vps33abf) mutation on a non-agouti (a/a) background have a lightened coat color that has been described as khaki. On an agouti background, Vps33abf homozygotes have dark ears, opaque eyes, and the belly is lighter in color than is the rest of the coat. Although no change in eye color is outwardly evident, electron microscopy shows fewer and smaller melanosomes than normal in the retinal pigment epithelium and choroid. These mutants have a platelet-storage pool defect evidenced by an increased bleeding time of 9.7 minutes on average, a reduction in the number of platelet dense granules, and slightly decreased collagen-mediated platelet aggregation, secretion of dense-granule ATP and secretion of seratonin. Hakansson and Lundin found increased activity of the lysosomal glycosidases beta- galactosidase, beta-glucuronidase, and N-acetyl-beta-hexosaminidase in kidney cell lysates. Suzuki et al. found no significant difference in kidney beta- galactosidase and beta-glucuronidase activity, no significant decrease in beta-glucuronidase secretion into the urine, but a 53% reduction in the secretion of beta-galactosidase into the urine. (Dickie, 1964; Davisson, 1977; Hakansson and Lundin, 1977; Suzuki et al., 2003.)
Mice homozygous for jg have shortened tails with anormal tapering tip. There is variation in expressivity. The tail is often kinked and other parts of the spine can also be affected. Testes are much smaller than normal and homozygous males are likely sterile. Ovaries are slightly smaller than normal and litter sizes from homozygous females are smaller than normal. Viability to wean age is reduced in homozygotes impacting males more than females. (Green, 1964.)
|Gene Symbol and Name||a, nonagouti|
|Strain of Origin||old mutant of the mouse fancy|
|General Note||Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039|
|Molecular Note||Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.|
|Gene Symbol and Name||Vps33a, VPS33A CORVET/HOPS core subunit|
|Strain of Origin||C57BL/6J|
|Molecular Note||This allele comprises a T-to-G transversion that causes a missense substitution of aspartic acid to glutamic acid at codon 251 (p.D251E).|
|Gene Symbol and Name||Micrl, microwave induced increase in complement receptor B cells|
|Strain of Origin||multiple strains|
|General Note||Carriers of this dominant QTL respond to microwave exposure with an increase in splenic C3 receptor-bearing B cells. The following inbred strains are homozygous for Micrl |
Homozygous jg/jg usually dies at birth
When using the JGBF/LeJ mouse strain in a publication, please include JAX stock #000260 in your Materials and Methods section.