Mice homozygous for the staggerer spontaneous mutation (Rorasg) show a staggering gait, mild tremor, hypotonia, and small size. The cerebellar cortex of homozygous mutant mice is grossly underdeveloped with a deficiency of granule cells and Purkinje cells. The remaining granule cells migrate inward from the external layer prematurely and then degenerate. Purkinje cells are much delayed in postnatal differentiation and lack the dendritic spines on which synapses with the parallel fibers from the granule cells normally occur. Staggerer mutant mice have been used as a source of an agranulate cerebellum in a number of investigations of the composition and function of granule cells. Kopmels et al. have reported a hyperproduction of IL1 biological activity and mRNA from LPS stimulated spleen cells of Rorasg/Rorasg mice on the C57BL/6J background relative to wild type siblings.
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | a | nonagouti |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Rora | RAR-related orphan receptor alpha |
Mice homozygous for the staggerer spontaneous mutation (Rorasg) show a staggering gait, mild tremor, hypotonia, and small size. The cerebellar cortex of homozygous mutant mice is grossly underdeveloped with a deficiency of granule cells and Purkinje cells. The remaining granule cells migrate inward from the external layer prematurely and then degenerate. Purkinje cells are much delayed in postnatal differentiation and lack the dendritic spines on which synapses with the parallel fibers from the granule cells normally occur. Staggerer mutant mice have been used as a source of an agranulate cerebellum in a number of investigations of the composition and function of granule cells. Kopmels et al. have reported a hyperproduction of IL1 biological activity and mRNA from LPS stimulated spleen cells of Rorasg/Rorasg mice on the C57BL/6J background relative to wild type siblings.
The first Rorasg/Rorasg mouse was observed in 1955 among the F2 progeny of a (BALB/cHm x C3H/HeJ)F1 female and a male of an obese(Lepob) stock of mixed background. The mutation was maintained for several generations by an intercross-backcross (within the same or a parallel lineage) mating scheme, then was backcrossed onto C57BL/6J for four generations. A Rorasg/+ male from the fourth backcross was mated to a female C57BL/10-Myo5ad Bmp5se mouse to introduce the dilute and short-ear mutations into the stock in repulsion with Rorasg; this allowed the heterozygotes (Rorasg + +/+ Myo5ad Bmp5se to be identified by a lack of either recessive phenotype. Brother-sister inbreeding was continued. In late 1978, at generation F53, a repulsion heterozygote, (Rorasg + +/+ Myo5ad Bmp5se) was outcrossed to a C3FeLe.B6-a/J and two of their Rorasg heterozygous offspring, which lacked Myo5ad and Bmp5se, were intercrossed to generate a homozygous female from which ovaries were transplanted and the host was bred to a B6C3FeF1/J-a/a male. This line was maintained for many years through this 2-generation breeding scheme, with a homozygous ovarian transplant host being bred to a B6C3FeF1/J-a/a male and then intercrossing their obligate Rorasg heterozygous offspring. In 2003 embryos were generated for cryopreservation from B6C3FeF1/J-a/a females bred to heterozygous males then at generation NE44F1, having been through 44 two-generation breeding cycles of crossing to the F1 hybrid.
Allele Name | nonagouti |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | a, nonagouti |
Gene Synonym(s) | |
Strain of Origin | old mutant of the mouse fancy |
Chromosome | 2 |
General Note | Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039 |
Molecular Note | Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type. |
Allele Name | staggerer |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | RORalpha-; sg |
Gene Symbol and Name | Rora, RAR-related orphan receptor alpha |
Gene Synonym(s) | |
Strain of Origin | obese stock |
Chromosome | 9 |
Molecular Note | This allele contains a 6.5kb genomic deletion of an exon encoding part of the ligand binding domain. The deletion results in an exon-skipping event that introduces a shift in the reading frame. The resulting protein is predicted to be truncated due to introduction of a premature stop codon. |
When using the staggerer mouse strain in a publication, please cite the originating article(s) and include JAX stock #000237 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for a, Heterozygous or wildtype for Rora<sg> |
Frozen Mouse Embryo | B6C3Fe a/a-Rora<sg>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6C3Fe a/a-Rora<sg>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6C3Fe a/a-Rora<sg>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6C3Fe a/a-Rora<sg>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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