The downless Jackson (Edardl-J) mutation arose spontaneously in a cross of two non-inbred silver (si) stocks in 1964. One silver stock was from Lane, of mixed genetic background including brown and nonagouti. The other silver stock was that of R. H. Schaible, which contained ae and Ames waltzer. An outcross was made from a downless Jackson mutant to C57BL/6J and sibling matings were made to F11. Backcrossing to C57BL/6J was again made for 5 generations by the cross-intercross method and again sibling matings for 5 generations. A cross was then made to C3HeB/Fe-a/a and following the first intercross the stock was mated to the B6C3Fe-a/a hybrid, a background on which it has been continued. It was cryopreserved in 1981 by mating homozygous males at N17 to B6C3Fe-a/a F1 females.
|Gene Symbol and Name||a, nonagouti|
|Strain of Origin||old mutant of the mouse fancy|
|General Note||Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039|
|Molecular Note||Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.|
|Allele Name||downless Jackson|
|Gene Symbol and Name||Edar, ectodysplasin-A receptor|
|Strain of Origin||STOCK Pmelsi|
|General Note||Similar phenotypes have also been described in mice for the Tabby allelic series at the Eda locus.|
|Molecular Note||This allele involves a G-to-A transition mutation at coding nucleotide 1135 that causes a glutamate to lysine change at position 379 (p.E379K).|
Homozygotes may develop tail lesions. Maloccluson is infrequent on this background.
When using the B6C3Fe a/a-Edardl-J/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000210 in your Materials and Methods section.