Hairs from mice homozygous for nonagouti (a) lack the yellow pigment band found in the agouti (A) coat color, leaving the coat a solid black color. In this congenic strain, C3HeB/FeJLe mice (normally agouti) have a black coat color.Read More +
Hairs from mice homozygous for nonagouti (a) lack the yellow pigment band found in the agouti (A) coat color, leaving the coat a solid black color. Although most hairs are non-banded (eumelanotic), hair found around the ears, genital papilla and mammae, are partly yellow. In this congenic strain, C3HeB/FeJLe mice (normally agouti) have a black coat color.
The C57BL/6J-derived nonagouti (a) loci on chromosome 2 was introgressed onto the C3HeB/FeJLe background for at 10 generations with selection for a at each generation.
|Gene Symbol and Name||a, nonagouti|
|Strain of Origin||old mutant of the mouse fancy|
|General Note||Insertion of the LV30 retrotransposon without the beta4 retrovirus sequence does not cause the nonagouti phenotype. J:278039|
|Molecular Note||Characterization of this allele shows an insertion of DNA comprised of a 5.5kb virus-like element, VL30, into the first intron of the agouti gene. The VL30 element itself contains an additional 5.5 kb sequence, flanked by 526 bp of direct repeats (beta4 retroviral sequence). The host integration site is the same as for at-2Gso and Aw-38J and includes a duplication of four nucleotides of host DNA and a deletion of 2 bp from the end of each repeat. Northern analysis of mRNA from skin of homozygotes shows a smaller agouti message and levels 8 fold lower than found in wild-type.|
When using the C3FeLe.B6-a/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #000198 in your Materials and Methods section.