B6By(CBy)-H2-D1^bm14/(HZW42)ByJ

Stock number: 000145
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

This T to A point substitution in H2-D1^b results in a glutamine to histidine single amino acid substitution in the peptide binding groove. This strain is valuable for characterizing the variation such a change causes in the array of bound antigenic peptides.

Detailed description

The H2-D1^bm14 allele is a spontaneous variant from the parental H2^b haplotype derived from C57BL/6By. This variant differs from the parental sequence by a single T to A nucleotide substitution causing a codon change from glutamine to histidine at amino acid 70 in the alpha 1 domain, which points into the peptide binding domain. Although C57BL/6J mice, homozygous for H2^b, are resistant to Daniel’s strain of Theiler’s murine encephalomyelitis virus, H2^bm14 homozygotes are not resistant to this virus and develop demyelinating lesions in the spinal cord subsequent to infection (Lipton et al., 1995, Block et al., 2005). The failure to mount a response results from failed antigen presentation (Block et al., 2005). These homozygotes are also unable generate an H2-D restricted response to Moloney leukemia virus or H-Y antigen as C57BL/6J mice do (Stukart et al., 1982 and de Waal et la., 1983) and heterozygotes are susceptible to Friend retrovirus-induced leukemia, whereas C57BL/6J is resistant (Miyazawa et al., 1991).

Development

The bm14 mutation arose spontaneously in HW42 (See stock No. 000340) when that congenic strain was at N14F22. This new mutant subline was designated HZW42 and the mutation named H2^bo or H2^bmo which was then re-named H2-D1^bm14. This strain was maintained by sibling mating until embryos were generated for cryopreservation by sibling mating in 1980 when this strain was at generation F40.

Allele

Symbol: H2-D1^bm14
Name: b haplotype mutation 14
MGI accession ID: MGI:3577203
Generation method: Spontaneous
Marker symbol: H2-D1
Marker name: histocompatibility 2, D region locus 1
Chromosome: 17
Strain of origin: C57BL/6By
Molecular note: This mutation on the H2^b haplotype is the result of a glutamine to histidine change at amino acid residue 70. Mice display a reduced expression of the H-2.2 specificity.
General note: Mutation arose in C57BL/6By congenic line Hw42.