This B6/By congenic strain carries the ‘minor’ histocompatibility allele, H24c, from BALB/cBy along with Gpi1a. The Gpi1 congenic mice are used as markers in transplant studies.
Don Bailey, The Jackson Laboratory
Genetic Background | Generation |
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000663 C57BL/6By |
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Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Gpi1 | glucose phosphate isomerase 1 |
Marker Symbol | Marker Name | |
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H24 | histocompatibility 24 |
This B6/By congenic strain carries the ‘minor’ histocompatibility allele, H24c, from BALB/cBy along with Gpi1a. The Gpi1 congenic mice are used as markers in transplant studies.
Dr. Donald Bailey, then at University of California Medical Center in San Francisco, began to generate a panel of congenic lines with differential loci that determine histocompatibility by crossing BALB/cBy females with C57BL/6By males, and then backcrossing at each generation to a C57BL/6J male. At backcross generation 5 orthotopic tailskin graft tests were done to identify lines bearing BALB/cBy-derived congenic intervals with histocompatibility loci. This backcrossing, with testing, was continued until siblings at backcross generation 11 or more were intercrossed to breed the congenic interval to homozygosity and each strain was maintained by sibling inbreeding (Bailey, 1975). Dr. Bailey transferred the mice to The Jackson Laboratory when he joined the staff in 1967. The strain was cryopreserved in 1980 at generation N16F42.
Allele Name | a variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | |
Gene Symbol and Name | Gpi1, glucose phosphate isomerase 1 |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 7 |
Molecular Note | This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains 101/H, A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1b. Two differences are silent, while a third difference at position nucleotide position 282 predicts an aspartate for the a allele and an asparagine for the b allele at amino acid position 94. A fourth difference in the 3' untranslated region was also noted. |
Marker Synonym(s) | |
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Chromosome(s) | 7 |
When using the HW54 mouse strain in a publication, please cite the originating article(s) and include JAX stock #000123 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for H24<c> 1 pair minimum |
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