C57BL/6J-Mc1r^e/J

Stock number: 000060
Research areas: Dermatology Research, Endocrine Deficiency Research

Development

The recessive yellow mutation (Mc1r^e) arose in 1963 in a C57BL/6Ha strain homozygous for Kit^w at Roswell Park Memorial Institute. By 1970 Dr. Elizabeth Russell had imported Mc1r^e on the C57BL/6Ha background into The Jackson Laboratory and began backcrossing it onto C57BL/6J. In 1974 the strain, maintained by backcross-intercross, had reached generation N8, in 1976 it reached generation N12, in 1978 it reached generation N18, and in 1979 it was cryopreserved at generation N20. The cryopreserved stocks were replenished in 2014 at generation N21F?pN1p (cryopreserved embryos from C57BL/6J oocytes fertilized by sperm from generation N21 homozygous males).

Allele

Symbol: Mc1r^e
Name: recessive yellow
MGI accession ID: MGI:1856028
Generation method: Spontaneous
Marker symbol: Mc1r
Marker name: melanocortin 1 receptor
Chromosome: 8
Strain of origin: C57BL/6Ha
Molecular note: The mutation is a frameshift due to a deletion of a single nucleotide at position 549. This mutation is predicted to result in a protein that continues out of frame for 12 amino acids before terminating.