FL/4ReJ

Stock number: 000025
Strain synonyms: FL/4Re-+/+ Lv^b/Lv^b
Research areas: Mouse/Human Gene Homologs, Sensorineural Research

Detailed description

This inbred strain carries the wild-type allele of the flexed-tail mutation. (See FL/1ReJ, Stock No. 000023).

Development

The initial FL/4Re inbred strain described in Coleman et al., 1968 was discontinued but restarted from the same FL/1Re segregating congenic line in 1970. This cryopreserved FL/4Re strain, which is also wildtype for f and Kit, and homozygous for the b polymorphism of Alad, was generated from FL/1Re mice at backcross generation N44 to N46, which were heterozygous for f, wildtype for Kit, and homozygous for the b polymorphism of Alad. Sibling intercrossing and selection for wildtype at the f locus yielded FL/4Re. This strain was also found to be homozygous for the Pde6b^rd1 allele. In 1979 embryos were generated for cryopreservation by sibling intercross at generation F27.

Allele

Symbol: Pde6b^rd1
Name: retinal degeneration 1
MGI accession ID: MGI:1856373
Generation method: Spontaneous
Marker symbol: Pde6b
Marker name: phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Chromosome: 5
Strain of origin: various
Molecular note: Two mutations have been identified in rd1 mice. A murine leukimia virus (Xmv-28) insertion in reverse orientation in intron 1 is found in all mouse strains with the rd1 phenotype. Further, a nonsense mutation (C-to-A transversion) in codon 347 that results in a truncation eliminating more than half of the predicted encoded protein, including the catalytic domain, has been identified in all rd1 strains of mice. A specific degradation of mutant transcript during or after pre-mRNA splicing is suggested.
General note: The following inbred strains are known to be homozygous for Pde6b: C3H sublines, CBA/J, FVB/NJ, PL/J, SB, SJL/J, and SWR/J.