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This assay cannot distinguish hemi from hom
Tg= 81 bp
IPC = 74 bp
Tg Sequence: aggacggctgcttcatctacaaggtgaagttcatcggcgtgaacttcccctccgacggccccgtgatgcagaagaagacca
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
37529 | AGG ACG GCT GCT TCA TCT AC | Transgene Forward | A | |||
37530 | TGG TCT TCT TCT GCA TCA CG | Transgene Reverse | A | |||
37531 | Fluorophore-1 | AGT TCA TCG GCG TGA ACT TC | Quencher-1 | Tg Probe | ||
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
37529 | 0.40 uM |
37530 | 0.40 uM |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
006055 | B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J |
006051 | B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J |
008705 | B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J |
006872 | B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J |
018410 | B6.Cg-Tg(Pnkd*A7V*A9V,-DsRed)671Ljp/J |
013082 | B6.FVB-Tg(Per2-DsRed*T3)12Obr/Mmjax |
006679 | B6;129P2-Or8a1tm27Mom/MomJ |
029332 | B6N.Cg-Tg(P2rx4-tdTomato)1Khakh/J |
037280 | C57BL/6-Tg(Irgm1-DsRed2)1Nci/Mmjax |
009655 | C57BL/6J-Tg(Dcx-DsRed)14Qlu/J |
005328 | NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J |
005438 | STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J |
005441 | STOCK Tg(CAG-DsRed*MST)1Nagy/J |
008241 | STOCK Tg(Cspg4-DsRed.T1)1Akik/J |
14 strains use this protocol |