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Mutant = T/T
Heterozygote = C/T
Wild type = C/C
X-linked mutation
TGTTTAGATCTCTAAATTACATAAACTTTTATTTACCTTCTTCTTGATATGAATGAAACT
CATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGT
TCTTTGAAAGAGCAA(c/t)AAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGA
TGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGA
TTGAGGGGCACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTA
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
16077 | ATT TTG AGG CTC TGC AAA GTT C | Forward | A | |||
16078 | GCC CCT CAA TCT CTT CAA ATT C | Reverse | A | |||
16079 | Fluorophore-1 | TGA AAG AGC AAC AAA ATG GCT TC | Quencher-1 | WT Probe | ||
16080 | Fluorophore-2 | TGA AAG AGC AAT AAA ATG GCT TC | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
16077 | 0.40 uM |
16078 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
017929 | B10.Cg-Cmahtm1Avrk Dmdmdx/PtmJ |
027818 | B10.Cg-Dmdmdx Tg(ACTA1-DMD*)326Dua/Mmjax |
027820 | B10.Cg-Dmdmdx Tg(Myh6-DMD*)#Dua/Mmjax |
027819 | B10.Cg-Dmdmdx Tg(Myh6-DMD*)47Dua/Mmjax |
018018 | B10ScSn.Cg-Prkdcscid Dmdmdx/J |
019014 | B10ScSn.Cg-Utrntm1Ked Dmdmdx/J |
027821 | B6.B10-Dmdmdx/Mmjax |
027822 | C.B10-Dmdmdx/Mmjax |
001801 | C57BL/10ScSn-Dmdmdx/J |
013141 | D2.B10-Dmdmdx/J |
022506 | D2.Cg-Utrntm1Ked Dmdmdx/J |
038371 | D2.Cg-Tg(DMD*)#Spc Dmdmdx/J |
027823 | FVB.Cg-Dmdmdx Tg(Myh6-DMD*)271Dua/Mmjax |
018915 | STOCK Terctm1Rdp Dmdmdx/J |
016622 | STOCK Utrntm1Jrs Dmdmdx/J |
014563 | STOCK Utrntm1Ked Dmdmdx/J |
16 strains use this protocol |