Protocol 19364 - Zc4h2-KOMP CRISPR

Notes

Mut = X

WT= 779bp

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Sequence

SEQ

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note
28571		AGG CAA TTC TAG CTA TGG GAG A		Forward
28572		CAC AGG GGT CCC AGT AAC A		Reverse

Strains Using This Protocol

This is the only strain that uses this protocol.