The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant = ~360 bp
Heterozygote = 270 bp and ~360 bp
Wild type = 270 bp
<p>Separated by gel electrophoresis on a 1.5% agarose gel.</p>
<p><span style="color: rgb(255, 0, 0);">NE</span></p>