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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR1093 | TGC TGA TGC CCT GGC CTG CT | Forward | ||||
oIMR1094 | TGG TCC CAC ATA TGC ACA TG | Reverse |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 1.00 X |
MgCl2 | 2.00 mM |
dNTP | 0.20 mM |
oIMR1093 | 0.80 uM |
oIMR1094 | 0.80 uM |
DNA Loading Dye | 0.69 mM |
Taq DNA Polymerase | 0.01 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 64.0 | -- | *-0.5 C per cycle |
4 | 72.0 | -- | repeat steps 2-4 for 12 cycles |
5 | 94.0 | -- | |
6 | 58.0 | -- | |
7 | 72.0 | -- | repeat steps 5-7 for 25 cycles |
8 | 72.0 | -- | |
9 | 10.0 | -- | hold** |
Component | Final Concentration |
---|---|
AB PCR BufferII | X |
ddH2O | |
BSA | mg/ml |
Fnu4HI |
Stock Number | Strain Name |
---|---|
012307 | B6.129-Apoetm3(APOE*4)Mae Ldlrtm1(LDLR)Mae/J |
006860 | B6.Cg-Ins2Akita Bdkrb2tm1Jfh/SmiJ |
006580 | B6.Cg-Ins2Akita Ldlrtm1Her/J |
004369 | B6.Cg-Rag1tm1Mom Ins2Akita/J |
003548 | C57BL/6-Ins2Akita/J |
007562 | D2.B6-Ins2Akita/MatbJ |
006867 | FVB.B6-Ins2Akita/MlnJ |
008659 | NOD.Cg-Rag1tm1Mom Ins2Akita Prf1tm1Sdz/SzJ |
014568 | NOD.Cg-Rag1tm1Mom Ins2Akita Il2rgtm1Wjl/SzJ |
9 strains use this protocol |