A ~2300 bp read-through band may be amplified from the mutant allele.
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant = 227 bp
Heterozygote = 227 bp and 470 bp
Wild type = 470 bp
Separated by gel electrophoresis on a 1.5% agarose gel.