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Mut = T H2<k> Jrs: 646, 656, 465, 4804
WT= C other haplotypes
tcccaccaTGAGCCTCTACCCTCTAGGAATTCG(c/t)CAGGGACCAGTCAGGTC
ACTCAGCTggggcttct
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
21918 | Fluorophore-1 | TAG GAA TTC GTC AGG GAC CAG | Quencher-1 | MUT Probe | ||
21919 | Fluorophore-2 | AGG AAT TCG CCA GGG ACC | Quencher-2 | WT Probe | ||
22189 | ATC CCA CCA TGA GCC TCT AC | Forward | A | |||
22190 | CAG CTG AGT GAC CTG ACT GG | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
22189 | 0.40 uM |
22190 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
000465 | B10.BR-H2k2 H2-T18a/SgSnJ |
004804 | B10.BR-H2k2 H2-T18a/SgSnJJrep |
036602 | B10.Cg-H2k2 H2-T18a Tg(SV40-HA)HA104Ajca/VradMmjax |
000666 | C57BL/10SnJ |
000486 | MRL/MpJ |
026243 | NOD.Cg-(D9rs4135590-D9rs13480186)H2k2Tg(ILK3mHEL)3Ccg Tg(TcrHEL3A9)1Mmd/SlsgJ |
6 strains use this protocol |