For in-depth product & services help, ask our
Technical Information Scientists
Taqman qPCR protocols are run on a real time PCR instrument. Use an appropriate instrument specific Fluorophore/Quencher combination.
>chr3:89651898+89651981 84bp CTCAAAGTCTTATACCATTCCCTTG CACACAGTTAGGAGGTAAAACACA
Mut= 114 bp
Wt= 84 bp
Fam=Mut
Hex=Wt
Wt Sequence:
acaagctttatggcatgtcttgaccaattagagactcaaagtcttataccattcccttgaaatgccaacactaagcctggggcacaccaagtcttgtgttttacctcctaactgtgtgttttctctcctgggaccctctgacgtcctctggcagGGTGCAGGCTGCAC
Mutant Sequence:
GGGCGCAAGCAGGTTTTGACAAGCTTTATGGCATGTCTTGACCAATTAGAGACTCAAAGTCTTATACCATTCCCTTGAAATGCCAACACTAAGCCTATAACTTCGTATAGCATACATTATACGAAGTTAtCACACCAAGTCTTGTGTTTTACCTCCTAACTGTGTGTTTTCTCTCCTGGGACCCTCTGACGTCCTCTGGCAGGGTGCAGGCTGCACTGCCCTTGTGG
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
56863 | CTC AAA GTC TTA TAC CAT TCC CTT G | Forward | A | |||
56864 | CAC ACA GTT AGG AGG TAA AAC ACA | Reverse | A | |||
56865 | Fluorophore-1 | AAG CCT GGG GCA CAC C | Quencher-1 | WT Probe | ||
56866 | Fluorophore-2 | CAC TAA GCC TAT AAC TTC GTA TAG CAT | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
56863 | 0.40 uM |
56864 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |