Protocol 46316: Sanger sequencing Assay - GET8305_Kcnh1_DEL-SEQ

C57BL/6J-Kcnh1^em1Lutzy/Mmjax

Stock No: 039011

Version 1.0

Notes

Mut = X

WT= Y

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Sequence

SEQ

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note
67687		ACC TGA ACC GCA AAG TGT TC		Wild type Forward
67688		CTG GTT AAA TGT GGC TGT CC		Wild type Reverse
67689		CTC CCA CAA GGT TCA CTC TGA		Mutant Forward
67690		CCC ATT TGA ATC CTG AAA CA		Mutant Reverse

Strains Using This Protocol

This is the only strain that uses this protocol.