For in-depth product & services help, ask our
Technical Information Scientists
Mutant = T/T, C/C, T/T, C/C, T/T
Heterozygote = A/T, G/C, G/T, A/C, A/T
Wild type = A/A, G/G, G/G, A/A, A/A
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
18879 | CCA CAC TCG CTG AGG TAT TTC | Forward | A | |||
18880 | GTC GAA GCG CAC GAA CTC | Reverse | A | |||
18881 | Fluorophore-1 | GTA CAT GGA AGT CGG CTA CGT G | Quencher-1 | WT Probe | ||
18882 | Fluorophore-2 | GTT CAT CTC TGT CGG TTA CGT G | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
18879 | 0.40 uM |
18880 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |