The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant (floxed) = 750 bp
Heterozygote = 750 bp and 550 bp
Wild type = 550 bp
To genotype for null allele (post cre excision) use
oIMR7911, AGG CGG ATT TCT GAG TTC GA with oIMR7912
Separated by gel electrophoresis on a 1.5% agarose gel.