The Col4a5 gene is X-LINKED. Males with the targeted allele are hemizygous mutants
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant (-/- or -/Y) = 158 bp
Heterozygote = 158 bp and 124 bp
Wild type (+/+ or +/Y) = 124 bp
Separated by gel electrophoresis on a 3.0% agarose gel.