The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant = 15 bp, 43 bp and 138 bp
Heterozygote = 15 bp, 43 bp, 138 bp and 153 bp
Wild type = 43 bp and 153 bp
uncut band = 196 bp
Separated by gel electrophoresis on a 3.0% agarose gel.**