These mice carry a targeted knock-out of the mouse Epn2 (epsin2) gene, useful in a range of research areas, including embryonic development and organogenesis.
Pietro De Camilli, Yale University School of Medicine, HHMI
These mice carry a targeted knock-out of the mouse Epn2 (epsin2) gene, useful in a range of research areas, including embryonic development and organogenesis. An absence of protein expression was confirmed by the donating laboratory through western blotting of multiple tissues from homozygous mice. Homozygous Epn2 knock-out mice demonstrate no overt phenotype.
Widespread knock-out of Epn1 (derived from Stock No. 031165) combined with a knock-out of Epn2 results in embryonic lethality at approximately E9.5.
Mice carrying both a knock-out of Epn2 and a vascular endothelial knock-out of Epn1, achieved via crosses with a tamoxifen-inducible Cdh5 (VEcadherin)-cre strain, demonstrate decreased tumor growth. A 50% reduced incidence at 1 week is observed in animals subcutaneously implanted mouse Lewis lung carcinoma (LLC) or melanoma cells. Reduced growth of gliomas, colorectal tumors, and prostate tumors have also been shown in compound mutant mice.
Triple knock-out mice derived from an Epn1 floxed line (see 031165), this Epn2 knock-out strain, and Epn3 knock-out mice (see Stock No. 014108) are also embryonic lethal. Their cells display a dramatic cell division defect and a robust impairment in clathrin-mediated endocytosis.
The 3' end of exon 1 and the 5' end of the following intron were replaced with a neomycin cassette via homologous recombination in 129Sv/J-derived embryonic stem cells. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
|Allele Name||targeted mutation 1, Ottavio Cremona|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Epn2, epsin 2|
|Strain of Origin||129X1/SvJ|
|Molecular Note||The 3' end of the first coding exon and the 5'end of the following intron were replaced with a neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in multiple tissues from homozygous mice.|
Homozygous males and females are viable and fertile.
When using the Epn2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #031166 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.