These mice carry a targeted knock-out of the mouse Epn2 (epsin2) gene, useful in a range of research areas, including embryonic development and organogenesis.
Pietro De Camilli, Yale University School of Medicine, HHMI
These mice carry a targeted knock-out of the mouse Epn2 (epsin2) gene, useful in a range of research areas, including embryonic development and organogenesis. An absence of protein expression was confirmed by the donating laboratory through western blotting of multiple tissues from homozygous mice. Homozygous Epn2 knock-out mice demonstrate no overt phenotype.
Widespread knock-out of Epn1 (derived from Stock No. 031165) combined with a knock-out of Epn2 results in embryonic lethality at approximately E9.5.
Mice carrying both a knock-out of Epn2 and a vascular endothelial knock-out of Epn1, achieved via crosses with a tamoxifen-inducible Cdh5 (VEcadherin)-cre strain, demonstrate decreased tumor growth. A 50% reduced incidence at 1 week is observed in animals subcutaneously implanted mouse Lewis lung carcinoma (LLC) or melanoma cells. Reduced growth of gliomas, colorectal tumors, and prostate tumors have also been shown in compound mutant mice.
Triple knock-out mice derived from an Epn1 floxed line (see 031165), this Epn2 knock-out strain, and Epn3 knock-out mice (see Stock No. 014108) are also embryonic lethal. Their cells display a dramatic cell division defect and a robust impairment in clathrin-mediated endocytosis.
The 3' end of exon 1 and the 5' end of the following intron were replaced with a neomycin cassette via homologous recombination in 129Sv/J-derived embryonic stem cells. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
|Allele Name||targeted mutation 1, Ottavio Cremona|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Epn2, epsin 2|
|Strain of Origin||129X1/SvJ|
|Molecular Note||The 3' end of the first coding exon and the 5'end of the following intron were replaced with a neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in multiple tissues from homozygous mice.|
Homozygous males and females are viable and fertile.
When using the Epn2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #031166 in your Materials and Methods section.