KLF6SV1 mice express a human KLF6-SV1 variant driven by the Col1a2 promoter in hepatic stellate cells. These mice may be useful when studying liver tumor suppressor gene function.
Scott L. Friedman, Mount Sinai School of Medicine
KLF6SV1 mice express a human Kruppel-like factor 6 (KLF6) gene driven by the collagen, type I, alpha 2 (Col1a2) promoter in hepatic stellate cells (HSC). KLF6 (Kruppel-like factor 6) is a zinc finger protein which acts as a transcriptional activator, and functions as a tumor suppressor. SV1, the major KLF6 splice variant, lacks the DNA binding domain, and has been associated with proliferation and facilitation of tumor invasion by antagonizing the transactivation of p21 and E-cadherin. It has pro-apoptotic caspase activity and accelerates the degradation of anti-apoptotic proteins. The stellate cell is the major cell type involved in liver fibrosis, which is the formation of scar tissue in response to liver damage. COL1A2 is activated as early as 12h after tissue injury. KLF6SV1 mice exhibit a slight decrease in fibrosis and in fibrogenic gene expression in fresh HSC isolates following 3 doses of carbon tetrachloride (CCl4). CCl4 induces damage by the formation of free radicals. Homozygous mice are viable and fertile. KLF6WT mice are more susceptible to apoptotic stress based on PARP cleavage.
The KLF6SV1 transgene was designed with the human variant isoform of the KLF6 gene driven by the collagen, type I, alpha 2 (Col1a2) promoter. KLF6-SV1 lacks the DNA binding domain of the KLF6 gene. The transgene was microinjected into fertilized C57BL/6 oocytes. Founder mice were bred to C57BL/6 mice and were subsequently backcrossed to C57BL/6 mice for many generations by the donating lab. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J (Stock No. 000664) oocytes.
|Expressed Gene||KLF6, Kruppel like factor 6, human|
|Site of Expression|
|Allele Name||transgene insertion 1, Scott Friedman|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||transgene insertion 1, Scott Friedman; Tg(Col1a2-KLF6*)1Fdmn|
|Gene Symbol and Name||Tg(Col1a2-KLF6*)1Fdmn, transgene insertion 1, Scott Friedman|
|Promoter||Col1a2, collagen, type I, alpha 2, mouse, laboratory|
|Expressed Gene||KLF6, Kruppel like factor 6, human|
|Strain of Origin||C57BL/6|
|Molecular Note||The transgene was designed with a mutant human KLF6 gene driven by the collagen, type I, alpha 2 (Col1a2) promoter. The KLF6 alternative splice isoform - splice variant 1 (SV1) - lacks the DNA binding domain.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Colα2(I)-KLF6SV1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #029323 in your Materials and Methods section.
|Hemizygous or non carrier for Tg(Col1a2-KLF6*)1Fdmn|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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