Exons 6 and 7 of the mouse Akap11 gene are flanked by loxP sites in this Akap11lox mutant allele. Cre-mediated excision of the floxed region results in a knockout of a protein that is integral to water reabsorption processes in the kidney.
John D. Scott, University of Washington
A-Kinase Anchoring Protein 220 (AKAP220) protein, encoded by the Akap11 (A kinase (PRKA) anchor protein 11) gene, is a ubiquitously-expressed vesicular- and membrane-associated anchoring protein. It has been implicated in the modulation of cytoskeletal signaling events, and is integral to water reabsorption processes in the kidney.
Exons 6 and 7 of the mouse Akap11 gene are flanked by loxP sites in this Akap11lox conditional allele. Cre-mediated excision of the floxed region results in a knockout of protein expression.
Western blot analysis indicates that homozygous floxed mice express normal levels of AKAP220 protein. Animals display no over phenotype, reproduce normally with expected Mendelian ratios, and live normal life spans.
A loxP site and FRT-flanked neomycin cassette were introduced to intron 5 of the mouse Akap11 gene and a loxP site was placed in intron 7 via homologous recombination in (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. A resultant chimeric male was bred to C57BL/6J females. The neomycin cassette was excised through crosses with B6(C3)-Tg(Pgk1-FLPo)10Sykr/J animals (see Stock No. 011065), leaving exons 6 and 7 flanked by loxP sites. This line was backcrossed to C57BL/6J for 6 generations by the donating laboratory.
|Allele Name||targeted mutation 1.1, John D Scott|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Akap11, A kinase (PRKA) anchor protein 11|
|Strain of Origin||(129S6/SvEvTac x C57BL/6NCrl)F1|
|Molecular Note||A loxP site and FRT-flanked neomycin cassette are introduced into intron 5 of the gene, a second loxP site is placed in intron 7. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 6 and 7 flanked by loxP sites.|
Heterozygous and homozygous mice are viable and fertile.
When using the Akap11lox mouse strain in a publication, please cite the originating article(s) and include JAX stock #028921 in your Materials and Methods section.