This knockout/reporter mutant of the Clcc1 (chloride channel CLIC-like 1) gene has been generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory. Clcc1 encodes a chloride ion channel.Read More +
This strain was generated by the Knockout Mouse Phenotyping Program (KOMP2) at The Jackson Laboratory using embryonic stem cells provided by the International Knockout Mouse Consortium. Clcc1 (chloride channel CLIC-like 1) encodes a chloride ion channel. A beta-galactosidase containing cassette generates a knockout first reporter allele for the Clcc1 gene (Skarnes et al. Nature 474,337-342;2011). Phenotype data is being generated by the JAX KOMP2 program and will be available to the public when complete from the International Mouse Phenotyping Consortium website.
For additional information on this allele, please see the International Knockout Mouse Consortium website.
The L1L2_Bact_P cassette was inserted at a position on Chromosome 3 upstream of the critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s). The critical exon(s) is/are thus flanked by loxP sites. The construct was introduced into C57BL/6N-Atm1Brd-derived JM8A3.N1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into B6(Cg)- Tyrc-2J/J (Stock No. 58) blastocysts. The resulting chimeric males were bred to C57BL/6NJ (Stock No. 005304) females and then to B6N.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 014094) to remove the floxed neomycin and critical exon sequences. Resulting offspring were bred to C57BL/6NJ mice to remove the cre-expressing transgene.
|Allele Name||targeted mutation 1b, Mouse Biology Program, University of California, Davis|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Allele Synonym(s)||Clcc1tm1b(KOMP)Mbp; targeted mutation 1b, Mouse Biology Program, University of California, Davis|
|Gene Symbol and Name||Clcc1, chloride channel CLIC-like 1|
|Gene Synonym(s)||Mclc; Mid-1-related chloride channel 1; MCLC; Mclc|
|Strain of Origin||C57BL/6N-Atm1Brd|
|Molecular Note||The L1L2_Bact_P cassette was inserted at position 108670225 of Chromosome 3 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 108671247. The critical exon(s) were thus flanked by loxP sites and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding a knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.|
Heterozygotes may be bred to C57BL/6NJ (Stock No. 005304) or wildtype littermates. The viability and fertility of the cre-excised knockout allele will be provided as mice are characterized on the: International Knockout Mouse Consortium website.
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