Overview

Also Known As:B6.129P2(Cg)-Hprt, HCRT-EGFP

These mice have the Ple111-EGFP transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human HCRT gene direct expression of EGFP.

Donating Investigator

Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Genetic overview

Genetic Background	Generation

Hprt^{tm15(Ple111-EGFP)Ems}

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Hprt	hypoxanthine guanine phosphoribosyl transferase

View Genetics

Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Details

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in a small cluster of neurons in the hypothalamus.

Genetics

Hprt^{tm15(Ple111-EGFP)Ems}

Allele Symbol: Hprt^{tm15(Ple111-EGFP)Ems}

Allele Name	targeted mutation 15, Elizabeth M Simpson
Allele Type	Targeted (Reporter)
Allele Synonym(s)	HCRT-EGFP; Hprt1^{tm15(mEMS1774)Ems}; Hprt1^{tm15(Ple111-EGFP;mEMS1774)Ems}; Ple111
Gene Symbol and Name	Hprt, hypoxanthine guanine phosphoribosyl transferase
Gene Synonym(s)
Promoter	HCRT, hypocretin neuropeptide precursor, human
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in a small cluster of neurons in the hypothalamus.
Strain of Origin	(B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Chromosome	X
General Note	Germ line transmission of mutant cell line mEMS1774 has been established.
Molecular Note	The Ple111-EGFP transgene (pEMS1417) was designed with the 544 bp Ple111 minipromoter (HCRT-A; derived from a subsection of the promoter from the human hypocretin (orexin) neuropeptide precursor (HCRT)) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human hypocretin (orexin) neuropeptide precursor (HCRT) directs expression of an enhanced green fluorescent protein (EGFP) to a small cluster of EGFP-positive neurons is found in the hypothalamus. EGFP-immunoreactivity fills the cytoplasm of these cells, highlighting the cell body as well as extensive dendritic processes. This label is very specific to the hypothalamus and not seen in any other cell populations in the brain. Double label immunocytochemistry shows that a majority of these EGFP-positive hypothalamic cells co-express with the HCRT (orexin) neuropeptide (the gene from which this MiniPromoter was designed).
Mutations Made By	Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Neurobiology Research
  - cell marker
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: glial cells
  - Tissue/Cell Markers: glial cells, oligodendrocytes, Schwann cells
  - Tissue/Cell Markers: neurons
- Fluorescent Proteins
Neurobiology Research
- Fluorescent protein expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Hprt^{tm15(Ple111-EGFP)Ems}/Y
B6.129P2(Cg)-Hprt/Mmjax

normal phenotype

no abnormal phenotype detected
- Normal - heterozygous females and hemizygous males are viable and fertile

References

Additional - Hprt^{tm15(Ple111-EGFP)Ems} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Gt(rosa)26sor Probe
- Standard PCR:Hprt1
- Genotyping resources and troubleshooting
Breeding Considerations

The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
- Additional Breeding and Husbandry Support
Citation
When using the HCRT-EGFP mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32927 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:B6.129P2(Cg)-Hprt, HCRT-EGFP

Donating Investigator

Genetic overview

Research Applications

Expression Data

Hprttm15(Ple111-EGFP)Ems

Allele Symbol: Hprttm15(Ple111-EGFP)Ems

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Hprttm15(Ple111-EGFP)Ems/YB6.129P2(Cg)-Hprt/Mmjax

normal phenotype

References

Additional - Hprttm15(Ple111-EGFP)Ems related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Hprt^{tm15(Ple111-EGFP)Ems}

Allele Symbol: Hprt^{tm15(Ple111-EGFP)Ems}

Genotype: Hprt^{tm15(Ple111-EGFP)Ems}/Y
B6.129P2(Cg)-Hprt/Mmjax

Additional - Hprt^{tm15(Ple111-EGFP)Ems} related