Cre is expressed from the Kcnc2 (potassium voltage gated channel, Shaw-related subfamily, member 2, "Kv3.2") promoter in these transgenic mice. When crossed with a lacZ reporter strain, expression is seen in specific subsets of thalamic nuclei and other locations in the brain.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Cre is expressed from the "Kv3.2" promoter in these transgenic mice. When crossed with a lacZ reporter strain, strong expression was seen in the rostral and midline thalamic nuclei (xiphoid, reunions, rhomboid, centromedial, intermediodorsal, paraventricular, paracentral, centrolateral, submedius, mediodorsal, lateral dorsal, anteromedial, suprageniculate, and Paratenial). Moderate expression was observed in other thalamic nuclei: anteroventral, anterodorsal, ventral lateral, ventral anterior, parafascicular, and posterior thalamic group. Weak expression was observed in the lateral posterior,dorsal lateral geniculate, medial geniculate, and ventral posterior thalamic nuclei.
Strong activity was observed also in ventral medial and dorsal medial hypothalamic nuclei, pontine reticular nucleus, mesencephalic fifth, and 12th cranial nerve nuclei. Moderate recombination was also present in the superficial piriform cortex, indusium griseum, dorsal endopiriform nucleus, medial and median preoptic hypothalamic nuclei, medial vistibular nucleus, ventral cochlear nucleus, nucleus of trapezoid body, and abducens nucleus. Very small numbers of neurons expressed in the lateral septum, locus coeruleus, substantia nigra par reticulata, and parabrachial nucleus.
Cre was inserted into the translational start site of the Kv3.2 (Kcnc2) gene. The transgenic vector was introduced to C57BL/6 zygotes. The current background involves C57BL/6, but could potentially carry other genetic contributions.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | When crossed with a lacZ reporter strain, strong expression is seen in the rostral and midline thalamic nuclei. Moderate expression is observed in anteroventral, anterodorsal, ventral lateral, ventral anterior, parafascicular, and posterior thalamic nuclei. Weak expression is observed in the lateral posterior, dorsal lateral geniculate, medial geniculate, and ventral posterior thalamic nuclei. |
Allele Name | transgene insertion K128, Susumu Tonegawa |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | K128-Cre; Kv3.2-Cre |
Gene Symbol and Name | Tg(Kcnc2-cre)K128Stl, transgene insertion K128, Susumu Tonegawa |
Gene Synonym(s) | |
Promoter | Kcnc2, potassium voltage gated channel, Shaw-related subfamily, member 2, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | When crossed with a lacZ reporter strain, strong expression is seen in the rostral and midline thalamic nuclei. Moderate expression is observed in anteroventral, anterodorsal, ventral lateral, ventral anterior, parafascicular, and posterior thalamic nuclei. Weak expression is observed in the lateral posterior, dorsal lateral geniculate, medial geniculate, and ventral posterior thalamic nuclei. |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | This transgene expresses cre under the control of the highly restricted thalamic promoter of Kcnc2. |
When using the STOCK Tg(Kcnc2-Cre)K128Stl/LetJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #008582 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(Kcnc2-Cre)K128Stl |
Frozen Mouse Embryo | STOCK Tg(Kcnc2-Cre)K128Stl/LetJ | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Kcnc2-Cre)K128Stl/LetJ | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Kcnc2-Cre)K128Stl/LetJ | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Kcnc2-Cre)K128Stl/LetJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.