NOD.B6-Ptprc^b/Dvs

Stock number: 005652
Strain synonyms: NOD.CD45.2

Description

This NOD congenic strain can be useful in transplant studies with the C57BL/6J-derived Ptprc^b variant of the pan leukocyte marker, commonly known as CD45.2 or Ly5.2, being detectable by flow cytometry or immunohistochemistry. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth.

Detailed description

This NOD congenic strain can be useful in transplant studies with the C57BL/6J-derived Ptprc^b variant of the pan leukocyte marker, commonly known as CD45.2 or Ly5.2, being detectable by flow cytometry or immunohistochemistry. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth.

Development

The Ptprc^b variant allele was backcrossed from C57BL/6J onto NOD/ShiLtDvs for 10 backcross generations using marker-assisted breeder selection to speed the host contribution to the congenic. Mice were then intercrossed to homozygosity and maintained by sibling breeding. At N10 the congenic interval had the NOD-derived sequence at D1Mit286, then C57BL/6J-derived sequence at D1Mit446 and D1Mit348, then NOD-derived sequence at D1Mit202 and D1Mit144. At generation N10F29 another 2 backcrosses to NOD/ShiLtDvs were done and the strain again made homozygous and sibling intercrossed, reaching generation N12F18 in 2022.

Allele

Symbol: Ptprc^b
Name: b variant
MGI accession ID: MGI:4819850
Generation method: Not Applicable
Synonyms: CD45.2; Ly5^b
Marker symbol: Ptprc
Marker name: protein tyrosine phosphatase, receptor type, C
Marker synonyms: B220; CD45; CD45R; GP180; IMD105; LCA; L-CA; LY5; Ly-5; Lyt-4; RT7; T200
Chromosome: 1
Strain of origin: Not Applicable
Site of expression: Widely expressed on all adaptive and innate immune cells.
Molecular note: Ptprc^b is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485).

Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341).